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TABLE I

Parameters of Morphology and Single Cell Responses by Suction Electrode


Wild type
mG/mG
Morphological parameters   
    OS length (μm) 15 ± 0.5 (10) 6.3 ± 0.3 (12)b 
    OS diameter (μm) 1.4 ± 0.1 (7) 1.4 ± 0.1 (6) 
Pigment content (pmol/retina) 410 ± 35 (4) 47 ± 3 (6)b 
Single cell recordings   
    rmax (pA) 8.1± 0.3 (22) 5.9 ± 0.5 (14)b 
    I0 (photons μm−220.7 ± 0.8 (22) 725 ± 50 (14)b 
    I0Ac(500) (R*, photoisomerizations/rod) 9.7 ± 0.4 (22) 39 ± 3 (14)b 
    Integration time (ms) 233 ± 9 (18) 191 ± 9 (14)a 
    Time to peak (ms) 147 ± 2 (18) 139 ± 5 (14) 
    Single photon response (pA) 0.66 ± 0.05 (17) 0.16 ± 0.02 (13)b 
    A0 (s−225 ± 1 (13) 7.0 ± 0.7 (6)b 

Wild type
mG/mG
Morphological parameters   
    OS length (μm) 15 ± 0.5 (10) 6.3 ± 0.3 (12)b 
    OS diameter (μm) 1.4 ± 0.1 (7) 1.4 ± 0.1 (6) 
Pigment content (pmol/retina) 410 ± 35 (4) 47 ± 3 (6)b 
Single cell recordings   
    rmax (pA) 8.1± 0.3 (22) 5.9 ± 0.5 (14)b 
    I0 (photons μm−220.7 ± 0.8 (22) 725 ± 50 (14)b 
    I0Ac(500) (R*, photoisomerizations/rod) 9.7 ± 0.4 (22) 39 ± 3 (14)b 
    Integration time (ms) 233 ± 9 (18) 191 ± 9 (14)a 
    Time to peak (ms) 147 ± 2 (18) 139 ± 5 (14) 
    Single photon response (pA) 0.66 ± 0.05 (17) 0.16 ± 0.02 (13)b 
    A0 (s−225 ± 1 (13) 7.0 ± 0.7 (6)b 

Values are means ± SEM. The number used to determine each parameter is given in parentheses. rmax is saturating photoresponse amplitude. I0 is flash intensity at 500 nm, which gives the half maximal response. Time to peak and integration time were estimated from responses whose amplitude are <0.2rmax and within the linear range. Integration time is the area of the dim flash response divided by its peak amplitude. The amplitude of the single photon response was obtained from ensemble variance-to-mean ratio of the dim flash response. A0 is a gain factor for the rising phase of phototransduction cascade determined by fitting the equation of Pugh and Lamb (1993) to multiple responses of each cell.

a

Difference from wild-type rods being statistically significant, P < 0.05.

b

Difference from wild-type rods being statistically significant, P < 0.005.

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