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TABLE II

Comparison of Steady-State Kinetic Parameters of D439N Activation by Substrates/Inhibitors


Km of substrate/ inhibitora
Imaxb (exchange mode)
Km for Na+, substrate-boundc
τslowd
 μM pA mM ms 
L-Glu 500 ± 350 −28 ± 7 >300 2,600 ± 700 
L-Asp 100 ± 20 −150 ± 40 90 ± 40 140 ± 70 
TBOA 3 ± 1 63 ± 37 – – 

Km of substrate/ inhibitora
Imaxb (exchange mode)
Km for Na+, substrate-boundc
τslowd
 μM pA mM ms 
L-Glu 500 ± 350 −28 ± 7 >300 2,600 ± 700 
L-Asp 100 ± 20 −150 ± 40 90 ± 40 140 ± 70 
TBOA 3 ± 1 63 ± 37 – – 
a

Apparent affinity for substrate/inhibitor in the exchange mode.

b

Imax was determined at saturating glutamate concentrations in the exchange mode.

c

Apparent affinity of Na+ to glutamate-bound transporter.

d

Time constant of rising phase induced by l-glutamate or l-aspartate in the forward transport mode.

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