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Table II

Characteristics of the Currents Mediated by mKv1.7 Mutant Channels Expressed in Xenopus Oocytes Determined by TEVC Measurements


Charge
V1/2
Slope

RT75

τInac

  mV mV n ms n ms n 
mKv1.7L 7.45 4.4 ± 1.4 15.4 ± 0.5 20 4.4 ± 0.5 12 181.8 ± 15.4 15 
mKv1.7S 5.12 −4.3 ± 2.9 13.3 ± 0.6 15 7.5 ± 0.7 12 643.2 ± 80.1 13 
H10K 8.28 −5.3 ± 1.2b 15.3 ± 1.0 7.2 ± 1.4a 334.9 ± 40.1c 
H21K 8.28 −10.1 ± 2.3c 12.2 ± 0.7b 11.0 ± 1.3c 225.3 ± 9.0 
H10KH21K 9.11 −14.5 ± 2.6c 11.0 ± 1.6b 7.6 ± 0.8b 230.4 ± 20.3 
H10Q 7.28 −5.1 ± 1.8c 17.1 ± 1.1 3.4 ± 0.2 394.3 ± 65.8c 
H21Q 7.28 −1.5 ± 1.7a 17.5 ± 0.7 3.44 ± 0.3 516.6 ± 71.4c 
H10QH21Q 7.12 −0.2 ± 1.2 17.8 ± 0.5 3.24 ± 0.2 632.5 ± 86.9c 
M33I 7.45 −4.1 ± 1.2b 17.2 ± 0.6 4.0 ± 0.5 244.2 ± 51.5 
M33G 7.45 −3.5 ± 2.1a 14.9 ± 0.9 4.7 ± 0.8 127.9 ± 19.7 
C37A 7.48 −2.2 ± 1.4b 16.6 ± 0.4 5.3 ± 0.7 232.7 ± 49.1 11 
C41A 7.48 −0.5 ± 1.2 17.0 ± 0.5 3.3 ± 0.3 76.0 ± 3.08c 
C43A 7.48 −0.1 ± 1.4 15.4 ± 0.5 4.5 ± 0.4 89.2 ± 4.9b 
C44A 7.48 −1.0 ± 1.7 17.7 ± 0.4 3.0 ± 0.1a 65.4 ± 10.8c 
Kv1.7S-H342G 5.12 −1.8 ± 3.1 16.5 ± 1.5 9.8 ± 1.0 737.6 ± 102.1 
Kv1.7S-C12A 5.12 −1.5 ± 1.0 14.7 ± 1.7 10.7 ± 1.8 1117.0 ± 38.4 

Charge
V1/2
Slope

RT75

τInac

  mV mV n ms n ms n 
mKv1.7L 7.45 4.4 ± 1.4 15.4 ± 0.5 20 4.4 ± 0.5 12 181.8 ± 15.4 15 
mKv1.7S 5.12 −4.3 ± 2.9 13.3 ± 0.6 15 7.5 ± 0.7 12 643.2 ± 80.1 13 
H10K 8.28 −5.3 ± 1.2b 15.3 ± 1.0 7.2 ± 1.4a 334.9 ± 40.1c 
H21K 8.28 −10.1 ± 2.3c 12.2 ± 0.7b 11.0 ± 1.3c 225.3 ± 9.0 
H10KH21K 9.11 −14.5 ± 2.6c 11.0 ± 1.6b 7.6 ± 0.8b 230.4 ± 20.3 
H10Q 7.28 −5.1 ± 1.8c 17.1 ± 1.1 3.4 ± 0.2 394.3 ± 65.8c 
H21Q 7.28 −1.5 ± 1.7a 17.5 ± 0.7 3.44 ± 0.3 516.6 ± 71.4c 
H10QH21Q 7.12 −0.2 ± 1.2 17.8 ± 0.5 3.24 ± 0.2 632.5 ± 86.9c 
M33I 7.45 −4.1 ± 1.2b 17.2 ± 0.6 4.0 ± 0.5 244.2 ± 51.5 
M33G 7.45 −3.5 ± 2.1a 14.9 ± 0.9 4.7 ± 0.8 127.9 ± 19.7 
C37A 7.48 −2.2 ± 1.4b 16.6 ± 0.4 5.3 ± 0.7 232.7 ± 49.1 11 
C41A 7.48 −0.5 ± 1.2 17.0 ± 0.5 3.3 ± 0.3 76.0 ± 3.08c 
C43A 7.48 −0.1 ± 1.4 15.4 ± 0.5 4.5 ± 0.4 89.2 ± 4.9b 
C44A 7.48 −1.0 ± 1.7 17.7 ± 0.4 3.0 ± 0.1a 65.4 ± 10.8c 
Kv1.7S-H342G 5.12 −1.8 ± 3.1 16.5 ± 1.5 9.8 ± 1.0 737.6 ± 102.1 
Kv1.7S-C12A 5.12 −1.5 ± 1.0 14.7 ± 1.7 10.7 ± 1.8 1117.0 ± 38.4 

Charge was determined at pH 7 for the N-terminal 182 aa or 150 aa in Kv1.7S. RT75 and τInac were determined at 40 mV.

a

P < 0.01, compared to mKv1.7L.

b

P < 0.001, compared to mKv1.7L.

c

P = 0.0001, compared to mKv1.7L.

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