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TABLE II

Conductance–Voltage Data for HCN2 Channels



Control

Saturating cAMP

HCN2 channel
 
Vhalf (mV)
 
Slope (mV)
 
Vhalf (mV)
 
Slope (mV)
 
ΔVhalf (mV)
 
Wild type (7) −125 ± 2.0 3.76 ± 0.30 −111 ± 1.7 4.18 ± 0.24 14.3 ± 1.8 
K472E (4) −113 ± 3.1 9.59 ± 0.89 −112 ± 2.9 9.06 ± 0.91 0.75 ± 1.3 
E502K (6) −128 ± 2.9 5.66 ± 0.97 −116 ± 2.8 5.4 ± 1.1 12.3 ± 0.94 
K472E + E502K (4) −125 ± 3.8 5.55 ± 0.82 −110 ± 3.4 5.72 ± 0.41 14.9 ± 1.6 
D542K (5) −124 ± 1.9 7.03 ± 0.61 −114 ± 1.9 6.35 ± 0.75 10.1 ± 1.4 
K472E + D542K (3) −133 ± 1.9 3.65 ± 0.57 −119 ± 3.6 3.52 ± 0.31 14.2 ± 1.9 
E502K + D542K (4)
 
−118 ± 2.8
 
7.58 ± 1.4
 
−122 ± 2.8
 
7.27 ± 1.1
 
−3.9 ± 0.95
 


Control

Saturating cAMP

HCN2 channel
 
Vhalf (mV)
 
Slope (mV)
 
Vhalf (mV)
 
Slope (mV)
 
ΔVhalf (mV)
 
Wild type (7) −125 ± 2.0 3.76 ± 0.30 −111 ± 1.7 4.18 ± 0.24 14.3 ± 1.8 
K472E (4) −113 ± 3.1 9.59 ± 0.89 −112 ± 2.9 9.06 ± 0.91 0.75 ± 1.3 
E502K (6) −128 ± 2.9 5.66 ± 0.97 −116 ± 2.8 5.4 ± 1.1 12.3 ± 0.94 
K472E + E502K (4) −125 ± 3.8 5.55 ± 0.82 −110 ± 3.4 5.72 ± 0.41 14.9 ± 1.6 
D542K (5) −124 ± 1.9 7.03 ± 0.61 −114 ± 1.9 6.35 ± 0.75 10.1 ± 1.4 
K472E + D542K (3) −133 ± 1.9 3.65 ± 0.57 −119 ± 3.6 3.52 ± 0.31 14.2 ± 1.9 
E502K + D542K (4)
 
−118 ± 2.8
 
7.58 ± 1.4
 
−122 ± 2.8
 
7.27 ± 1.1
 
−3.9 ± 0.95
 

All values are mean ± SEM. The n values are in parentheses after each channel construct. ΔVhalf = Vhalf cAMP − Vhalf control.

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