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TABLE I

Screening for Inward Rectification Properties of Mutants




No alteration of rectification

No functional currents
N-cytoplasmic region G65R, A70R, A70K, A70S, R74A R80Q C54S, D71E, D78E 
Pore region A91T, V93I, V93T, F163V, I166V, G168T, I176T S136F 
C-cytoplasmic region
 
K223Q, I201V, L231E, S238K, E293Q, A306S, R325E, E333S, D340K, E349K, K362E, E387
 
L217E, E303K, E303Q, G300V
 



No alteration of rectification

No functional currents
N-cytoplasmic region G65R, A70R, A70K, A70S, R74A R80Q C54S, D71E, D78E 
Pore region A91T, V93I, V93T, F163V, I166V, G168T, I176T S136F 
C-cytoplasmic region
 
K223Q, I201V, L231E, S238K, E293Q, A306S, R325E, E333S, D340K, E349K, K362E, E387
 
L217E, E303K, E303Q, G300V
 

The amino acid sequence of Kir 2.1 was compared with that of sWIRK. To survey critical sites for inward rectification, these point mutants were made in the wild-type and D172N & E224G & E299Q triple mutant of Kir 2.1.

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