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TABLE I

Summary of the Values of Passive Force, Maximal Active Force, pCa50, ΔpCa50, and nH in Control PLV, sTn-reconstituted PLV, and Control Rabbit Psoas Muscles


SL
Passive force
Maximal force
pCa50
ΔpCa50
nH
 μmN/mm2 mN/mm2    
Fig. 1 C        
    PLV 1.9 ∼0 54.89 ± 2.24 5.56 ± 0.01  3.83 ± 0.20 
 2.3 10.11 ± 0.53 70.25 ± 3.87 5.81 ± 0.02 0.24 ± 0.01 3.11 ± 0.29 
    PLV (+sTn) 1.9 ∼0 51.43 ± 2.07 (54.36 ± 2.80) 5.79 ± 0.01a  3.20 ± 0.20 
 2.3 10.23 ± 0.82 58.26 ± 3.58a 5.93 ± 0.02a 0.14 ± 0.02a 3.01 ± 0.10 
    Rabbit psoas 1.9 ∼0 161.72 ± 8.91ab 5.89 ± 0.03ab  4.14 ± 0.22 
 2.3 3.80 ± 0.76ab 178.68 ± 8.74ab 6.01 ± 0.02ab 0.12 ± 0.01a 3.40 ± 0.27 
Control experiments       
    PLV (+cTn) 1.9 ∼0 50.70 ± 3.15 (54.17 ± 4.50) 5.60 ± 0.01  3.28 ± 0.11 
 2.3 7.90 ± 2.57 67.73 ± 3.70 5.82 ± 0.05 0.23 ± 0.04 2.60 ± 0.11 
    Rabbit psoas (+sTn) 1.9 ∼0 129.40 ± 14.02 (129.35 ± 10.96) 5.86 ± 0.03  4.21 ± 0.31 
 2.3 3.53 ± 0.52 152.58 ± 17.78 5.97 ± 0.04 0.11 ± 0.01 4.42 ± 0.55 

SL
Passive force
Maximal force
pCa50
ΔpCa50
nH
 μmN/mm2 mN/mm2    
Fig. 1 C        
    PLV 1.9 ∼0 54.89 ± 2.24 5.56 ± 0.01  3.83 ± 0.20 
 2.3 10.11 ± 0.53 70.25 ± 3.87 5.81 ± 0.02 0.24 ± 0.01 3.11 ± 0.29 
    PLV (+sTn) 1.9 ∼0 51.43 ± 2.07 (54.36 ± 2.80) 5.79 ± 0.01a  3.20 ± 0.20 
 2.3 10.23 ± 0.82 58.26 ± 3.58a 5.93 ± 0.02a 0.14 ± 0.02a 3.01 ± 0.10 
    Rabbit psoas 1.9 ∼0 161.72 ± 8.91ab 5.89 ± 0.03ab  4.14 ± 0.22 
 2.3 3.80 ± 0.76ab 178.68 ± 8.74ab 6.01 ± 0.02ab 0.12 ± 0.01a 3.40 ± 0.27 
Control experiments       
    PLV (+cTn) 1.9 ∼0 50.70 ± 3.15 (54.17 ± 4.50) 5.60 ± 0.01  3.28 ± 0.11 
 2.3 7.90 ± 2.57 67.73 ± 3.70 5.82 ± 0.05 0.23 ± 0.04 2.60 ± 0.11 
    Rabbit psoas (+sTn) 1.9 ∼0 129.40 ± 14.02 (129.35 ± 10.96) 5.86 ± 0.03  4.21 ± 0.31 
 2.3 3.53 ± 0.52 152.58 ± 17.78 5.97 ± 0.04 0.11 ± 0.01 4.42 ± 0.55 

Passive force was obtained at the steady state. PLV (+sTn), sTn-reconstituted PLV. Control experiments, muscles treated with the Tn complex identical to the endogenous one (see text). PLV (+cTn), cTn-reconstituted PLV (n = 5); Rabbit psoas (+sTn), sTn-reconstituted rabbit psoas muscle (n = 6). (P > 0.05 compared with corresponding values for original muscles, except for nH at SL 1.9 μm in PLV [+cTn]). Maximal force was obtained by activating muscle at pCa 4.5 prior to construction of the force–pCa curve at each SL (passive force was measured just prior to activation at pCa 4.5). Numbers in parentheses indicate maximal force values obtained prior to reconstitution (P > 0.05 compared with corresponding values obtained after reconstitution).

a

P < 0.05 compared with corresponding values for control PLV.

b

P < 0.05 compared with corresponding values for sTn-reconstituted PLV.

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