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Table 2.

Apoptosis analysis of Gata2 E11 AGM and FL cells

Genotype/apoptotic status AGM FL 
 Flk1+cKit- endothelial cells Flk1+cKit+ cluster base cells Flk1-cKit+ hematopoietic cells CD31+cKit+ HPCs/HSCs CD31-cKit+ hematopoietic cells 
 % % % % % 
Gata2f/+ or Gata2f/f (WT) Total: 2.0 ± 0.6 Total: 2.3 ± 0.6 Total: 19.2 ± 3.4 Total: 60.2 ± 4.2 Total: 32.4 ± 4.2 
 Viable 76 76 77 75 
 Early apoptotic 13 
 Late apoptotic 21 
 Dead 14 12 11 76 
Vec-Cre:Gata2f/f Total: 1.5 ± 0.3 Total: 2.1 ± 0.7 Total: 17.0 ± 2.3 Total: 22.1 ± 0.4** Total: 68.6 ± 1.4** 
 Viable 76 74 74 30** 
 Early apoptotic 15 15 
 Late apoptotic 20 18** 
 Dead 14 12 35 80** 
Genotype/apoptotic status AGM FL 
 Flk1+cKit- endothelial cells Flk1+cKit+ cluster base cells Flk1-cKit+ hematopoietic cells CD31+cKit+ HPCs/HSCs CD31-cKit+ hematopoietic cells 
 % % % % % 
Gata2f/+ or Gata2f/f (WT) Total: 2.0 ± 0.6 Total: 2.3 ± 0.6 Total: 19.2 ± 3.4 Total: 60.2 ± 4.2 Total: 32.4 ± 4.2 
 Viable 76 76 77 75 
 Early apoptotic 13 
 Late apoptotic 21 
 Dead 14 12 11 76 
Vec-Cre:Gata2f/f Total: 1.5 ± 0.3 Total: 2.1 ± 0.7 Total: 17.0 ± 2.3 Total: 22.1 ± 0.4** Total: 68.6 ± 1.4** 
 Viable 76 74 74 30** 
 Early apoptotic 15 15 
 Late apoptotic 20 18** 
 Dead 14 12 35 80** 

Results of flow cytometric analyses with various cell subset specific markers (Flk1, cKit, and CD31) show the percentage of cells within total populations of E11 AGM or E11 FL (Total). Apoptotic status of cells within these gated populations (cell subsets) was tested by Annexin V and Hoechst staining and is shown below the percentage of the total population. Viable cells are Annexin VHoechst, early apoptotic are Annexin V+Hoechst, late apoptotic are Annexin V+Hoechst+, and dead cells are Annexin VHoechst+. n = 3. Numbers of embryos analysed = 7 WT and 7 Vec-Cre:Gata2f/f. Significance was determined by Student’s t test. **, P < 0.01.

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