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Table III.

Histological analysis of the infarcted area

	WT 7d (n = 8)	SPARC-null, 7d (n = 7)	WT + TGF-β, 7d (n = 6)	SPARC-null + TGF-β, 7d (n = 6)	WT, 14 d (n = 10)	SPARC-null, 14 d (n = 11)
Infarct size (%)	43 ± 3.6	46 ± 4.1	38 ± 3	36 ± 4	42 ± 2.6	43 ± 2.7
Residual necrotic area (%)	9.6 ± 1.8	9.6 ± 2.2	14.3 ± 6.9	3.9 ± 1.7	2.7 ± 0.7	1.0 ± 0.3^a
Infarct thickness (µm)	380 ± 61	290 ± 22	430 ± 40	560 ± 72^b	390 ± 27	380 ± 27
Leukocyte infiltration (cells/mm²)	870 ± 180	820 ± 140	720 ± 180	870 ± 80	660 ± 130	270 ± 68^a
Coronary growth (vessels/mm²)	42 ± 4	45 ± 5	59 ± 5^b	59 ± 3^b	54 ± 10	43 ± 5
Capillary growth (vessels/mm²)	89 ± 14	119 ± 18	135 ± 31	143 ± 23	87 ± 16	116 ± 13
Myofibroblast (% area)	2.1 ± 0.4	3.8 ± 0.8^a	1.7 ± 0.1	1.1 ± 0.2^b	0.8 ± 0.4	1.6 ± 0.2
Collagen deposition (%)	26 ± 2.5	20 ± 3.6	28 ± 2	30 ± 2^b	36 ± 2.4	37 ± 2.0
Ratio O-R thick/Y-G thin collagen fibers	11 ± 2.3	2.5 ± 0.5^a	20 ± 2.9^b	4.5 ± 1.2^b	17 ± 1.8	8.1 ± 1.8^a

	WT 7d (n = 8)	SPARC-null, 7d (n = 7)	WT + TGF-β, 7d (n = 6)	SPARC-null + TGF-β, 7d (n = 6)	WT, 14 d (n = 10)	SPARC-null, 14 d (n = 11)
Infarct size (%)	43 ± 3.6	46 ± 4.1	38 ± 3	36 ± 4	42 ± 2.6	43 ± 2.7
Residual necrotic area (%)	9.6 ± 1.8	9.6 ± 2.2	14.3 ± 6.9	3.9 ± 1.7	2.7 ± 0.7	1.0 ± 0.3^a
Infarct thickness (µm)	380 ± 61	290 ± 22	430 ± 40	560 ± 72^b	390 ± 27	380 ± 27
Leukocyte infiltration (cells/mm²)	870 ± 180	820 ± 140	720 ± 180	870 ± 80	660 ± 130	270 ± 68^a
Coronary growth (vessels/mm²)	42 ± 4	45 ± 5	59 ± 5^b	59 ± 3^b	54 ± 10	43 ± 5
Capillary growth (vessels/mm²)	89 ± 14	119 ± 18	135 ± 31	143 ± 23	87 ± 16	116 ± 13
Myofibroblast (% area)	2.1 ± 0.4	3.8 ± 0.8^a	1.7 ± 0.1	1.1 ± 0.2^b	0.8 ± 0.4	1.6 ± 0.2
Collagen deposition (%)	26 ± 2.5	20 ± 3.6	28 ± 2	30 ± 2^b	36 ± 2.4	37 ± 2.0
Ratio O-R thick/Y-G thin collagen fibers	11 ± 2.3	2.5 ± 0.5^a	20 ± 2.9^b	4.5 ± 1.2^b	17 ± 1.8	8.1 ± 1.8^a

O-R, orange-red; Y-G, yellow-green.

P < 0.05 in SPARC-null versus WT infarcts at corresponding time after MI.

P < 0.05 in TGF-β–treated infarcts versus untreated infarcts at the corresponding time after MI.