Fast Peptide Block of LqTx-modified Sodium Channels
| Peptide . | . | τ(0 mV) . | . | Fss(0 mV) . | . | k b(0 mV) . | . | k u(0 mV) . | . | z . | . | n . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ms | s−1 | s−1 | ||||||||||
| LqTx | 4.1 ± 0.7 | 3 | ||||||||||
| ac-RIFMR-NH2 | 0.28 ± 0.01 | 0.06 ± 0.01 | 3348 ± 100 | 222 ± 33 | 2+ | 3 | ||||||
| ac-KIFMK-NH2 | 0.35 ± 0.04 | 0.21 ± 0.03 | 2402 ± 308 | 587 ± 40 | 2+ | 7 | ||||||
| ac-KIFMTK-NH2 | 0.28 ± 0.02 | 0.20 ± 0.01 | 2881 ± 263 | 731 ± 8 | 2+ | 3 | ||||||
| KIFMT-NH2 | 0.67 ± 0.11 | 0.25 ± 0.01 | 1168 ± 181 | 386 ± 81 | 2+ | 3 | ||||||
| KIFM-NH2 | 0.33 ± 0.03 | 0.42 ± 0.01 | 1751 ± 163 | 1283 ± 149 | 2+ | 3 | ||||||
| IFM-NH(CH2)2-NH2 | 0.32 ± 0.02 | 0.25 ± 0.01 | 2367 ± 152 | 782 ± 105 | 2+ | 3 |
| Peptide . | . | τ(0 mV) . | . | Fss(0 mV) . | . | k b(0 mV) . | . | k u(0 mV) . | . | z . | . | n . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ms | s−1 | s−1 | ||||||||||
| LqTx | 4.1 ± 0.7 | 3 | ||||||||||
| ac-RIFMR-NH2 | 0.28 ± 0.01 | 0.06 ± 0.01 | 3348 ± 100 | 222 ± 33 | 2+ | 3 | ||||||
| ac-KIFMK-NH2 | 0.35 ± 0.04 | 0.21 ± 0.03 | 2402 ± 308 | 587 ± 40 | 2+ | 7 | ||||||
| ac-KIFMTK-NH2 | 0.28 ± 0.02 | 0.20 ± 0.01 | 2881 ± 263 | 731 ± 8 | 2+ | 3 | ||||||
| KIFMT-NH2 | 0.67 ± 0.11 | 0.25 ± 0.01 | 1168 ± 181 | 386 ± 81 | 2+ | 3 | ||||||
| KIFM-NH2 | 0.33 ± 0.03 | 0.42 ± 0.01 | 1751 ± 163 | 1283 ± 149 | 2+ | 3 | ||||||
| IFM-NH(CH2)2-NH2 | 0.32 ± 0.02 | 0.25 ± 0.01 | 2367 ± 152 | 782 ± 105 | 2+ | 3 |
The time course of peptide-induced decay of sodium currents was fit to Eq. 1, and the best fit parameters for the indicated number of experiments were averaged to yield the parameters presented.