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Table III

Inactivation Properties of Cysteine Mutants

Position-mutationTau inactivation (−10 mV)Steady state inactivationRecovery from inactivation (−110 mV)
KdSlope
  n  ms  mV  mV  ms 
WT  (17)  0.56 ± 0.04  −81.7 ± 2.1  5.6 ± 0.2   3.8 ± 0.3 
I1461-C   (7)  0.44 ± 0.01  −90.6 ± 2.7*   6.1 ± 0.3  10.5 ± 2.9** 
R1462-C   (9)  0.43 ± 0.03*  −82.6 ± 2.3  7.2 ± 0.2   2.1 ± 0.1** 
T1463-C   (5)  0.52 ± 0.02  −91.3 ± 2.4*  6.5 ± 0.5   4.4 ± 0.2 
L1464-C   (6)  0.95 ± 0.12*  −84.2 ± 2.7  9.2 ± 0.8   7.2 ± 1.3** 
L1465-C   (6)  0.76 ± 0.04*  −71.2 ± 3.3**  6.7 ± 0.5   1.8 ± 0.1** 
F1466-C   (4)  0.78 ± 0.16  −68.1 ± 3.6**  7.4 ± 0.3   2.0 ± 0.2** 
A1467-C   (6)  0.67 ± 0.1  −90.4 ± 3.0*  6.4 ± 0.3   6.3 ± 1.0** 
L1468-C   (5)  0.64 ± 0.09  −91.1 ± 2.1  8.1 ± 0.3   4.1 ± 0.4 
M1469-C   (6)  0.90 ± 0.05**  −75.6 ± 2.0  5.7 ± 0.4   3.5 ± 0.5 
M1470-C   (7)  0.52 ± 0.5  −87.4 ± 2.3   6.2 ± 0.4   6.1 ± 0.4** 
S1471-C   (5)  0.50 ± 0.1  −91.0 ± 2.9*   5.7 ± 0.3   8.0 ± 1.7** 
L1472-C   (7)  0.52 ± 0.06  −85.1 ± 3.2  5.0 ± 0.7   3.9 ± 0.8 
P1473-C  (14)  NR        
A1474-C   (5)  0.60 ± 0.03  −79.3 ± 1.8  5.3 ± 0.2   3.4 ± 0.4 
L1475-C   (5)  0.37 ± 0.02*  −76.8 ± 10.5  7.5 ± 1.0   1.8 ± 0.4* 
F1476-C   (7)  0.47 ± 0.03  −80.6 ± 1.8  7.0 ± 0.4   3.0 ± 0.2 
N1477-C   (8)  NR        
Position-mutationTau inactivation (−10 mV)Steady state inactivationRecovery from inactivation (−110 mV)
KdSlope
  n  ms  mV  mV  ms 
WT  (17)  0.56 ± 0.04  −81.7 ± 2.1  5.6 ± 0.2   3.8 ± 0.3 
I1461-C   (7)  0.44 ± 0.01  −90.6 ± 2.7*   6.1 ± 0.3  10.5 ± 2.9** 
R1462-C   (9)  0.43 ± 0.03*  −82.6 ± 2.3  7.2 ± 0.2   2.1 ± 0.1** 
T1463-C   (5)  0.52 ± 0.02  −91.3 ± 2.4*  6.5 ± 0.5   4.4 ± 0.2 
L1464-C   (6)  0.95 ± 0.12*  −84.2 ± 2.7  9.2 ± 0.8   7.2 ± 1.3** 
L1465-C   (6)  0.76 ± 0.04*  −71.2 ± 3.3**  6.7 ± 0.5   1.8 ± 0.1** 
F1466-C   (4)  0.78 ± 0.16  −68.1 ± 3.6**  7.4 ± 0.3   2.0 ± 0.2** 
A1467-C   (6)  0.67 ± 0.1  −90.4 ± 3.0*  6.4 ± 0.3   6.3 ± 1.0** 
L1468-C   (5)  0.64 ± 0.09  −91.1 ± 2.1  8.1 ± 0.3   4.1 ± 0.4 
M1469-C   (6)  0.90 ± 0.05**  −75.6 ± 2.0  5.7 ± 0.4   3.5 ± 0.5 
M1470-C   (7)  0.52 ± 0.5  −87.4 ± 2.3   6.2 ± 0.4   6.1 ± 0.4** 
S1471-C   (5)  0.50 ± 0.1  −91.0 ± 2.9*   5.7 ± 0.3   8.0 ± 1.7** 
L1472-C   (7)  0.52 ± 0.06  −85.1 ± 3.2  5.0 ± 0.7   3.9 ± 0.8 
P1473-C  (14)  NR        
A1474-C   (5)  0.60 ± 0.03  −79.3 ± 1.8  5.3 ± 0.2   3.4 ± 0.4 
L1475-C   (5)  0.37 ± 0.02*  −76.8 ± 10.5  7.5 ± 1.0   1.8 ± 0.4* 
F1476-C   (7)  0.47 ± 0.03  −80.6 ± 1.8  7.0 ± 0.4   3.0 ± 0.2 
N1477-C   (8)  NR        

Results show mean ± SEM. Number of analyzed cells are shown in parenthesis. Inactivation time constants (Tau) were obtained by single exponential fitting of current traces obtained from voltage pulses to −10 mV (inward current) and to +60 mV (outward current) from a holding potential of −120 mV. Results of Boltzmann fit of steady state inactivation curves are shown. Mutants that showed a significant difference compare to WT in a t test at the level of 0.05 are marked with one asterisk and at the level of 0.01 with two asterisks.  

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