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Table II

Inactivation Kinetics of D4/S4-5 Mutants

Position-mutationTau inactivationSteady state inactivationRecovery from inactivation (−110 mV)
KdSlope
  n  ms  mV  mV  ms 
WT  (17)  0.56 ± 0.04  −81.7 ± 2.1  5.6 ± 0.2   3.6 ± 0.1 
R1462-E  (10)  0.59 ± 0.06  −86.0 ± 2.5  10.0 ± 0.7   1.9 ± 0.2** 
R1462-K  (14)  0.53 ± 0.05  −81.9 ± 1.8  6.2 ± 0.3   2.7 ± 0.2** 
R1462-L   (6)  0.61 ± 0.05  −82.5 ± 2.8  8.6 ± 0.2   2.8 ± 0.1 
T1463-E   (4)  0.31 ± 0.05*  −93.4 ± 2.9**  7.1 ± 0.6   3.0 ± 0.4 
F1466-E   (7)   1.8 ± 0.2**  −66.8 ± 1.5**  7.6 ± 0.9   2.0 ± 0.2** 
F1466-W   (4)  0.56 ± 0.04  −84.4 ± 2.4  5.4 ± 0.3   4.5 ± 0.2 
L1468-A   (9)  0.43 ± 0.02*  −77.8 ± 2.5  5.6 ± 0.1   2.7 ± 0.3 
L1468-F  (10)  0.64 ± 0.05  −88.8 ± 2.4*  7.8 ± 0.4   3.6 ± 0.4 
L1468-M   (7)  0.66 ± 0.05  −80.0 ± 2.5  6.7 ± 0.3   3.2 ± 0.2 
L1468-S   (9)  0.66 ± 0.04  −74.5 ± 1.5  7.2 ± 0.4   1.7 ± 0.1** 
M1469-E   (4)   2.3 ± 0.2**  −51.2 ± 2.8**  11.7 ± 2.8   1.4 ± 0.2** 
M1469-K   (5)   2.6 ± 0.5**  −64.9 ± 7.5**  10.2 ± 1.4   1.5 ± 0.5** 
M1469-L   (4)  0.46 ± 0.06  −78.9 ± 2.7  4.7 ± 0.2   4.2 ± 0.4 
M1470-E   (9)   2.3 ± 0.2**  −66.0 ± 3.0**  7.4 ± 0.9   1.8 ± 0.1** 
M1470-F   (4)  0.41 ± 0.03  −86.4 ± 3.4  7.0 ± 0.3   2.9 ± 0.7 
M1470-K   (7)   1.7 ± 0.1**  −67.4 ± 5.3**  5.2 ± 0.1   1.4 ± 0.1** 
M1470-L  (10)  0.47 ± 0.05  −92.8 ± 2.9**  6.4 ± 0.4   8.1 ± 0.8** 
S1471-A   (5)  0.43 ± 0.06  −88.5 ± 1.2  5.4 ± 0.4   4.5 ± 0.4 
S1471-T   (8)  0.66 ± 0.05  −80.2 ± 3.5  6.6 ± 0.5   4.1 ± 0.4 
L1472-A   (9)  0.64 ± 0.04  −72.4 ± 1.3*  4.5 ± 0.3   3.3 ± 0.3 
L1472-F   (6)  0.69 ± 0.01  −71.2 ± 1.7*  5.7 ± 0.3   3.0 ± 0.3 
L1472-T   (8)  0.58 ± 0.02  −79.9 ± 1.2  6.1 ± 0.3   4.3 ± 0.4 
A1474-D  (10)   2.9 ± 0.3**  −60.2 ± 2.8**  8.0 ± 1.0   2.5 ± 0.2* 
A1474-K   (8)  0.65 ± 0.07  −70.2 ± 2.2**  6.1 ± 0.7   1.4 ± 0.1** 
L1475-E   (9)  0.73 ± 0.07*  −75.5 ± 4.4  7.5 ± 0.1   2.5 ± 0.3* 
L1475-F   (4)  0.38 ± 0.06  −78.8 ± 3.4  7.2 ± 0.5   1.9 ± 0.2* 
F1476-E   (9)  0.59 ± 0.06  −106.7 ± 2.3**  7.2 ± 0.7   7.5 ± 1.8** 
F1476-K   (6)  0.94 ± 0.12**  −92.2 ± 1.8**  8.8 ± 1.9  18.1 ± 4.2** 
F1476-W   (5)  0.41 ± 0.06  −93.3 ± 4.5*  7.2 ± 1.2   5.2 ± 0.8 
Position-mutationTau inactivationSteady state inactivationRecovery from inactivation (−110 mV)
KdSlope
  n  ms  mV  mV  ms 
WT  (17)  0.56 ± 0.04  −81.7 ± 2.1  5.6 ± 0.2   3.6 ± 0.1 
R1462-E  (10)  0.59 ± 0.06  −86.0 ± 2.5  10.0 ± 0.7   1.9 ± 0.2** 
R1462-K  (14)  0.53 ± 0.05  −81.9 ± 1.8  6.2 ± 0.3   2.7 ± 0.2** 
R1462-L   (6)  0.61 ± 0.05  −82.5 ± 2.8  8.6 ± 0.2   2.8 ± 0.1 
T1463-E   (4)  0.31 ± 0.05*  −93.4 ± 2.9**  7.1 ± 0.6   3.0 ± 0.4 
F1466-E   (7)   1.8 ± 0.2**  −66.8 ± 1.5**  7.6 ± 0.9   2.0 ± 0.2** 
F1466-W   (4)  0.56 ± 0.04  −84.4 ± 2.4  5.4 ± 0.3   4.5 ± 0.2 
L1468-A   (9)  0.43 ± 0.02*  −77.8 ± 2.5  5.6 ± 0.1   2.7 ± 0.3 
L1468-F  (10)  0.64 ± 0.05  −88.8 ± 2.4*  7.8 ± 0.4   3.6 ± 0.4 
L1468-M   (7)  0.66 ± 0.05  −80.0 ± 2.5  6.7 ± 0.3   3.2 ± 0.2 
L1468-S   (9)  0.66 ± 0.04  −74.5 ± 1.5  7.2 ± 0.4   1.7 ± 0.1** 
M1469-E   (4)   2.3 ± 0.2**  −51.2 ± 2.8**  11.7 ± 2.8   1.4 ± 0.2** 
M1469-K   (5)   2.6 ± 0.5**  −64.9 ± 7.5**  10.2 ± 1.4   1.5 ± 0.5** 
M1469-L   (4)  0.46 ± 0.06  −78.9 ± 2.7  4.7 ± 0.2   4.2 ± 0.4 
M1470-E   (9)   2.3 ± 0.2**  −66.0 ± 3.0**  7.4 ± 0.9   1.8 ± 0.1** 
M1470-F   (4)  0.41 ± 0.03  −86.4 ± 3.4  7.0 ± 0.3   2.9 ± 0.7 
M1470-K   (7)   1.7 ± 0.1**  −67.4 ± 5.3**  5.2 ± 0.1   1.4 ± 0.1** 
M1470-L  (10)  0.47 ± 0.05  −92.8 ± 2.9**  6.4 ± 0.4   8.1 ± 0.8** 
S1471-A   (5)  0.43 ± 0.06  −88.5 ± 1.2  5.4 ± 0.4   4.5 ± 0.4 
S1471-T   (8)  0.66 ± 0.05  −80.2 ± 3.5  6.6 ± 0.5   4.1 ± 0.4 
L1472-A   (9)  0.64 ± 0.04  −72.4 ± 1.3*  4.5 ± 0.3   3.3 ± 0.3 
L1472-F   (6)  0.69 ± 0.01  −71.2 ± 1.7*  5.7 ± 0.3   3.0 ± 0.3 
L1472-T   (8)  0.58 ± 0.02  −79.9 ± 1.2  6.1 ± 0.3   4.3 ± 0.4 
A1474-D  (10)   2.9 ± 0.3**  −60.2 ± 2.8**  8.0 ± 1.0   2.5 ± 0.2* 
A1474-K   (8)  0.65 ± 0.07  −70.2 ± 2.2**  6.1 ± 0.7   1.4 ± 0.1** 
L1475-E   (9)  0.73 ± 0.07*  −75.5 ± 4.4  7.5 ± 0.1   2.5 ± 0.3* 
L1475-F   (4)  0.38 ± 0.06  −78.8 ± 3.4  7.2 ± 0.5   1.9 ± 0.2* 
F1476-E   (9)  0.59 ± 0.06  −106.7 ± 2.3**  7.2 ± 0.7   7.5 ± 1.8** 
F1476-K   (6)  0.94 ± 0.12**  −92.2 ± 1.8**  8.8 ± 1.9  18.1 ± 4.2** 
F1476-W   (5)  0.41 ± 0.06  −93.3 ± 4.5*  7.2 ± 1.2   5.2 ± 0.8 

Results show mean ± SEM. Number of analyzed cells is shown in parenthesis. Inactivation time constants were obtained by single exponential fitting of current traces obtained from voltage pulses to −10 mV from a holding potential of −120 mV. Results of Boltzmann fit of steady state inactivation curves are shown. Mutants that showed a significant difference compare with WT in a t test at the level of 0.05 are marked with one asterisk and at the level of 0.01 with two asterisks.  

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