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Table I.

Change in α4β2 stoichiometry in the presence of nicotine (0.1 µM for 48 h)

β2 subunit Whole cell section TG / TGN 
wt 0.51 vs. 0.73 0.36 vs. 0.83 
L349M 0.70 vs. 0.79 0.69 vs. 0.82 
365AAQA368 0.67 vs. 0.70 0.63 vs. 0.74 
β2 subunit Whole cell section TG / TGN 
wt 0.51 vs. 0.73 0.36 vs. 0.83 
L349M 0.70 vs. 0.79 0.69 vs. 0.82 
365AAQA368 0.67 vs. 0.70 0.63 vs. 0.74 

Note that effects of nicotine and of β-subunit ER export mutations appear to approach a common value of increased (α4)2(β2)3 stoichiometry. NFRET measurements were fitted to Gaussian components, as in Fig. 5, yielding the total pixel area under either the A1 (lower NFRET) or A2 (higher NFRET) components. These components correspond, respectively, to (α4)2(β2)3 and (α4)3(β2)2 stoichiometry. Each number is the ratio of areas, A1/(A1 + A2). The entries denote control solution versus nicotine exposure in either the whole cell section or the Golgi apparatus (TG/TGN).

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