Rockefeller University Press

Table IV.

κ/λ L chain double-expressing clones from fusion 10 wk after cGvH (Bm-12 into 56R/B6)

Clone no.	56R PCR	Vκ(S)/J5	λ1 PCR	λ2 PCR	λx PCR	IgM ELISA	κ ELISA	λ1,2,3 ELISA	dsDNA ELISA  [OD]	MBP ELISA  [OD]	PS ELISA  [OD]
30	+	J5	+		+	+	+	+	1.284	1.919	0.274
35	+	J5	+		+	+	+	+	1.669	2.154	0.536
41	+	J5	+			+	+	+	1.741	2.006	0.768
124	+	J5	+		+	+	+	+	1.361	1.846	0.842
57	+			+		+		+	0.979	0.36	0.904
59	-		+				+	+	n.d.	n.d.	n.d.

Clone no.	56R PCR	Vκ(S)/J5	λ1 PCR	λ2 PCR	λx PCR	IgM ELISA	κ ELISA	λ1,2,3 ELISA	dsDNA ELISA  [OD]	MBP ELISA  [OD]	PS ELISA  [OD]
30	+	J5	+		+	+	+	+	1.284	1.919	0.274
35	+	J5	+		+	+	+	+	1.669	2.154	0.536
41	+	J5	+			+	+	+	1.741	2.006	0.768
124	+	J5	+		+	+	+	+	1.361	1.846	0.842
57	+			+		+		+	0.979	0.36	0.904
59	-		+				+	+	n.d.	n.d.	n.d.

n.d., not done.