Single cell analysis of B cells from the MZ of a 56R/B6 mouse
| A . | . | . | . | . | . |
|---|---|---|---|---|---|
| Single cells (179) . | 56R tg+ (124) . | Vκ(S) (124) . | Vκ38C (100) . | Vκ21D . | Vλ (26) . |
| Plate I | 67 | 80 | 30 | 11 | 15 |
| Plate II | 57 | 44 | 70 | n.d. | 11 |
| 56R tg+ (124) | Vκ (S) (87) | Vκ38C (64) | Vκ21D | Vλ (17) | |
| Plate I | 58 | 29 | 10 | 9 | |
| Plate II | 29 | 35 | n.d. | 8 |
| A . | . | . | . | . | . |
|---|---|---|---|---|---|
| Single cells (179) . | 56R tg+ (124) . | Vκ(S) (124) . | Vκ38C (100) . | Vκ21D . | Vλ (26) . |
| Plate I | 67 | 80 | 30 | 11 | 15 |
| Plate II | 57 | 44 | 70 | n.d. | 11 |
| 56R tg+ (124) | Vκ (S) (87) | Vκ38C (64) | Vκ21D | Vλ (17) | |
| Plate I | 58 | 29 | 10 | 9 | |
| Plate II | 29 | 35 | n.d. | 8 |
| B . | . | . | . | . | . |
|---|---|---|---|---|---|
| Cell no. . | 56R . | Vκ(S)/Cκ . | Vκ38C/Cκ . | Vκ21D/Cκ . | Vλ1/Cλ . |
| Plate I | |||||
| 10 | + | +a | |||
| 18 | +a | ||||
| 20 | + (Vκ21-4 (98%)/J2 (100%) IF) | +b (IF) | |||
| 22 | + | + | +a | ||
| 40 | + | + | |||
| 43 | + | + | + | ||
| 53 | + | + (Vκkk4 (91%)/J5 (95%) IF) | + (Vκ38C/J5 IF) | + (Vλ1/Jλ1/Cλ1 IF) | |
| 59 | + | + | +a | ||
| 62 | + | + | + (Vκ38C/J4 IF) | + (Vλ1/Jλ3/Cλ2 OF) | |
| 63 | + | + (Vκ21-4 (98%)/J2 (100%) IF) | + (Vκ38C/J5 IF) | + (Vλ1/Jλ1/Cλ1 OF) | |
| 74 | + | + (Vλ2/Jλ2/Cλ2 OF) | |||
| 75 | + (Vκ21-4 (98%)/J1 (100%) IF) | + | + (n.d.) | ||
| 76 | + | + | +a | ||
| 95 | + | + | + (Vλ1/Jλ1/Cλ1 OF) | ||
| Plate II | |||||
| 13 | + (Vκ21-4 (98%)/J2 (100%) IF) | n.d. | + (Vλ1/Jλ1/Cλ1 IF) | ||
| 14 | + | + (Vκ19-23 (98%)/J5 (100%) IF) | n.d. | + (Vλ1/Jλ1/Cλ1 OF) | |
| 29 | + | + (Vκ21-4%) (98)/J2 (97%) IF) | n.d. | + (Vλ1/Jλ3/Cλ? OF) | |
| 33 | + | + (Vκ19-15 (92%)/J5 (100%) OF) | + (Vκ38C/J4 IF) | n.d. | + (Vλ1/Jλ3/Cλ2 OF) |
| 34 | + | + (Vκ38C/J4 IF) | n.d. | + | |
| 49 | + | + (Vκ38C/J5 IF) | n.d. | + (Vλ1/Jλ1/Cλ1 OF) | |
| 52 | + | + (Vκ19-23 (98%)/J2 (100%) IF) | n.d. | + (Vλ1/Jλ1/Cλ1 OF) | |
| 59 | + | + (Vκ21-4 (98%)/J2 (96%) IF) | + (Vκ38C/J4 IF) | n.d. | +a (Vλ1) |
| 60 | + (Vκkk4 (99%)/J5 (100%) IF) | + (Vκ38C/J4 IF) | n.d. | + (Vλ1/Jλ1/Cλ1 IF) | |
| 71 | + | + (Vκ19-23 (97%)/J2 (100%) IF) | + (Vκ38C/J4 IF) | n.d. | + |
| B . | . | . | . | . | . |
|---|---|---|---|---|---|
| Cell no. . | 56R . | Vκ(S)/Cκ . | Vκ38C/Cκ . | Vκ21D/Cκ . | Vλ1/Cλ . |
| Plate I | |||||
| 10 | + | +a | |||
| 18 | +a | ||||
| 20 | + (Vκ21-4 (98%)/J2 (100%) IF) | +b (IF) | |||
| 22 | + | + | +a | ||
| 40 | + | + | |||
| 43 | + | + | + | ||
| 53 | + | + (Vκkk4 (91%)/J5 (95%) IF) | + (Vκ38C/J5 IF) | + (Vλ1/Jλ1/Cλ1 IF) | |
| 59 | + | + | +a | ||
| 62 | + | + | + (Vκ38C/J4 IF) | + (Vλ1/Jλ3/Cλ2 OF) | |
| 63 | + | + (Vκ21-4 (98%)/J2 (100%) IF) | + (Vκ38C/J5 IF) | + (Vλ1/Jλ1/Cλ1 OF) | |
| 74 | + | + (Vλ2/Jλ2/Cλ2 OF) | |||
| 75 | + (Vκ21-4 (98%)/J1 (100%) IF) | + | + (n.d.) | ||
| 76 | + | + | +a | ||
| 95 | + | + | + (Vλ1/Jλ1/Cλ1 OF) | ||
| Plate II | |||||
| 13 | + (Vκ21-4 (98%)/J2 (100%) IF) | n.d. | + (Vλ1/Jλ1/Cλ1 IF) | ||
| 14 | + | + (Vκ19-23 (98%)/J5 (100%) IF) | n.d. | + (Vλ1/Jλ1/Cλ1 OF) | |
| 29 | + | + (Vκ21-4%) (98)/J2 (97%) IF) | n.d. | + (Vλ1/Jλ3/Cλ? OF) | |
| 33 | + | + (Vκ19-15 (92%)/J5 (100%) OF) | + (Vκ38C/J4 IF) | n.d. | + (Vλ1/Jλ3/Cλ2 OF) |
| 34 | + | + (Vκ38C/J4 IF) | n.d. | + | |
| 49 | + | + (Vκ38C/J5 IF) | n.d. | + (Vλ1/Jλ1/Cλ1 OF) | |
| 52 | + | + (Vκ19-23 (98%)/J2 (100%) IF) | n.d. | + (Vλ1/Jλ1/Cλ1 OF) | |
| 59 | + | + (Vκ21-4 (98%)/J2 (96%) IF) | + (Vκ38C/J4 IF) | n.d. | +a (Vλ1) |
| 60 | + (Vκkk4 (99%)/J5 (100%) IF) | + (Vκ38C/J4 IF) | n.d. | + (Vλ1/Jλ1/Cλ1 IF) | |
| 71 | + | + (Vκ19-23 (97%)/J2 (100%) IF) | + (Vκ38C/J4 IF) | n.d. | + |
Single cells from the MZ (B220+CD21high and CD23low) in a 56R/B6 mouse were analyzed by PCR for Igκ, Igλ, and 56R H chain. All PCR product-positive cells were included in the table (A). B cells contained Igλ+ cells. B cells with in-frame λ L chain show message for one or two in-frame κ L chain(s) (in bold) (B). For further details see Table S3. Numbers in parentheses indicate sequence similarity to the germline gene in percentages.
n.d., not done.
IF, in frame.
OF, out of frame.
%, sequence similarity in percent, determined for κ L chains amplified with the degenerate primer V κ (S).
Deleterious events in V and J.
Deleterious events in V.