Binding of MBP and PS of 56R+IgM+ B cell hybridomas with different L chains
| A . | . | . |
|---|---|---|
| Vκ-gene . | MBP/dsDNA binding . | PS/dsDNA binding . |
| Vκ20/J2 | 0.77 ± 0.10 (n = 7) | 5.55 ± 1.78 (n = 3) |
| Vκ20/J4 | 0.58 ± 0.05 (n = 14) | 3.62 ± 1.02 (n = 6) |
| Vκ38c/J2 | 0.61 ± 0.09 (n = 7) | 0.40 ± 0.12 (n = 3) |
| Vκ38c/J4 | 0.92 ± 0.06 (n = 7) | 0.51 ± 0.27 (n = 3) |
| Vκ38c/J5 | 0.35 ± 0.03 (n = 11) | 1.06 ± 0.24 (n = 4) |
| Vκ(S)/J1 | 1.03 ± 0.07 (n = 7) | 0.25 ± 0.02 (n = 3) |
| B | ||
| Vκ-gene | MBP/dsDNA binding | PS/dsDNA binding |
| Vκ20/J2 | 1.46 ± 0.05 (n = 3) | 14.26 ± 0.85 (n = 3) |
| Vκ20/J4 | 0.85 ± 0.08 (n = 7) | 10.68 ± 2.41 (n = 7) |
| Vκ38c/J5 | 1.36 ± 0.14 (n = 3) | 1.25 ± 0.06 (n = 3) |
| A . | . | . |
|---|---|---|
| Vκ-gene . | MBP/dsDNA binding . | PS/dsDNA binding . |
| Vκ20/J2 | 0.77 ± 0.10 (n = 7) | 5.55 ± 1.78 (n = 3) |
| Vκ20/J4 | 0.58 ± 0.05 (n = 14) | 3.62 ± 1.02 (n = 6) |
| Vκ38c/J2 | 0.61 ± 0.09 (n = 7) | 0.40 ± 0.12 (n = 3) |
| Vκ38c/J4 | 0.92 ± 0.06 (n = 7) | 0.51 ± 0.27 (n = 3) |
| Vκ38c/J5 | 0.35 ± 0.03 (n = 11) | 1.06 ± 0.24 (n = 4) |
| Vκ(S)/J1 | 1.03 ± 0.07 (n = 7) | 0.25 ± 0.02 (n = 3) |
| B | ||
| Vκ-gene | MBP/dsDNA binding | PS/dsDNA binding |
| Vκ20/J2 | 1.46 ± 0.05 (n = 3) | 14.26 ± 0.85 (n = 3) |
| Vκ20/J4 | 0.85 ± 0.08 (n = 7) | 10.68 ± 2.41 (n = 7) |
| Vκ38c/J5 | 1.36 ± 0.14 (n = 3) | 1.25 ± 0.06 (n = 3) |
56R+ IgM hybridomas are compared from two fusions. Clones were polyreactive to at least two antigens in ELISA assays testing for dsDNA, PS, and MBP. The mean of the MBP/dsDNA and PS/dsDNA was calculated from at least three clones, and ± indicates the error (SEM). The values show the binding to PS and MBP relative to DNA binding. Fusion panels are from 4 (A) and 10 wk (B) after GvH. For more details see Table S2.