B cell hybridomas generated from 56R/B6 splenocytes 4 wk after cGvH reaction
| A . | . | . | . | . | . |
|---|---|---|---|---|---|
| Total clones
(78) . | 56R tg+
(33) . | . | . | . | . |
| IgM+ (29) | 24 | ||||
| Reactivity to | |||||
| dsDNA | 23 | ||||
| PS | 18 | ||||
| MBP | 21 | ||||
| IgG+ (24) | 9 | ||||
| Reactivity to | |||||
| dsDNA | 3 | ||||
| PS | 2 | ||||
| MBP | 3 | ||||
| B | |||||
| Total clones
(36) | Vκ12-13
(3) | Vκ20
(10) | Vκ21D
(4) | Vκ38C
(8) | Vκ(S)
(17) |
| 56R tg+ (25) | 1(J2)a 2(J5)a | 3(J2)a 5(J4)a | 4(J2)a | 1(J2) 1(J4) 4(J5)a | 9 |
| 56R tg−b (11) | 2(J5)a | 1(J2)a 1(J4) | 8 |
| A . | . | . | . | . | . |
|---|---|---|---|---|---|
| Total clones
(78) . | 56R tg+
(33) . | . | . | . | . |
| IgM+ (29) | 24 | ||||
| Reactivity to | |||||
| dsDNA | 23 | ||||
| PS | 18 | ||||
| MBP | 21 | ||||
| IgG+ (24) | 9 | ||||
| Reactivity to | |||||
| dsDNA | 3 | ||||
| PS | 2 | ||||
| MBP | 3 | ||||
| B | |||||
| Total clones
(36) | Vκ12-13
(3) | Vκ20
(10) | Vκ21D
(4) | Vκ38C
(8) | Vκ(S)
(17) |
| 56R tg+ (25) | 1(J2)a 2(J5)a | 3(J2)a 5(J4)a | 4(J2)a | 1(J2) 1(J4) 4(J5)a | 9 |
| 56R tg−b (11) | 2(J5)a | 1(J2)a 1(J4) | 8 |
56R+ clones from fusion 4 wk after cGvH (Bm-12 into 56R/B6) are polyreactive (A). κ L chain usage in 56R+ and 56R− clones from fusion 4 wk after cGvH (Bm-12 into 56R/B6). Only clones with H and L chain secretion are listed and were tested for rearrangement of κ L chains using specific primers for Vκ12-13, Vκ20, Vκ21D, and Vκ38C, and a degenerate primer Vκ(S) for other κ rearrangements (B). For more details see Table S1.
Groups that contain clones with two L chain rearrangements.
56R H chain transgenic−/CDR3JHCH+.
J, Jκ gene in rearrangement.