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Table II.

Number of mAbs generated by immunization with irradiated sporozoites or αDEC-CS plus maturation stimulus


Immunization method

Number of screened clonesa

Number of positive clones
 (% of the total screened)

Positive clones (%)b
   Major repeat Minor repeat 
Irradiated sporozoitesc 1,277 10 (0.8) 100 
αDEC-CS+anti-CD40+polyI:Cd
 
736
 
121 (16.4)
 
55.4
 
44.6
 

Immunization method

Number of screened clonesa

Number of positive clones
 (% of the total screened)

Positive clones (%)b
   Major repeat Minor repeat 
Irradiated sporozoitesc 1,277 10 (0.8) 100 
αDEC-CS+anti-CD40+polyI:Cd
 
736
 
121 (16.4)
 
55.4
 
44.6
 
a

Growing clones were screened for antibody secretion.

b

Growing clones were screened for reactivity against sequences representing the major repeat (QGPGAP)2 or the minor repeat (QQPP)2 of CSP.

c

Prime and boost were performed i.v. with 7.5 × 104 irradiated sporozoites in the interval of a month. The fusion was performed 3 d after the boost dose.

d

Prime i.p. with 10 μg αDEC-CS plus anti-CD40 plus poly I:C. 21 wk later, the boost was performed i.p. with 10 μg αDEC-CS. The fusion was performed 3 d after the boost dose.

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