Absence of TSLPR blocks the development of lung inflammation and infiltration of inflammatory cells
. | Analysis . | Score . | Monocytes . | Lymphocytes . | Neutrophils . | Eosinophils . |
|---|---|---|---|---|---|---|
| Normal lungs
Normal lungs
High perivascular
and peribronchial inflammation
Slight inflammation | % | % | % | % | ||
| WT + PBS | 0–1 | 87 ± 4 | 13 ± 4 | 0 | 0 | |
| KO + PBS | 0–1 | 95 ± 3 | 5 ± 3 | 0 | 0 | |
| WT + OVA | 3–3.5 | 42 ± 2 | 21 ± 3 | 16 ± 4 | 21 | |
| KO + OVA | 0.5–1.5 | 87 ± 2 | 11 ± 2 | 1 ± 1 | 1 ± 1 |
. | Analysis . | Score . | Monocytes . | Lymphocytes . | Neutrophils . | Eosinophils . |
|---|---|---|---|---|---|---|
| Normal lungs
Normal lungs
High perivascular
and peribronchial inflammation
Slight inflammation | % | % | % | % | ||
| WT + PBS | 0–1 | 87 ± 4 | 13 ± 4 | 0 | 0 | |
| KO + PBS | 0–1 | 95 ± 3 | 5 ± 3 | 0 | 0 | |
| WT + OVA | 3–3.5 | 42 ± 2 | 21 ± 3 | 16 ± 4 | 21 | |
| KO + OVA | 0.5–1.5 | 87 ± 2 | 11 ± 2 | 1 ± 1 | 1 ± 1 |
BALB/c WT and TSLPR KO mice were sensitized (i.p.) and challenged (i.t. and i.n.) with OVA or PBS (i.p.). Shown are levels of inflammation in the lungs observed in Fig. 4 by periodic acid-Schiff staining, as well as the distribution of infiltrating leukocytes in BAL. TSLPR KO mice that were challenged with OVA showed weak inflammation with much less infiltration of inflammatory cells than did WT animals. The increase in eosinophils and neutrophils was significant (P < 0.0001). The experiment was done four times with two to four mice in each experimental group in each experiment.