Phenotype of CD45−/− NK cells
Marker . | Surface expression in vivo . | . | |
|---|---|---|---|
| . | CD45+/+ . | CD45−/− . | |
| % positive | |||
| CD122 | 90.4 ± 0.9 | 88.4 ± 1.4 | |
| Ly49A | 13.0 ± 0.5 | 11.6 ± 0.8 | |
| Ly49C/I | 42.2 ± 6.5 | 37.2 ± 1.2 | |
| Ly49D | 45.0 ± 3.0 | 32.4 ± 4.3a | |
| Ly49G2 | 39.4 ± 1.1 | 39.4 ± 2.7 | |
| Ly49H | 47.6 ± 2.8 | 44.6 ± 3.3 | |
| Ly49F | 98.4 ± 0.8 | 10.0 ± 1.3 | |
| CD49b | 100 ± 0.0 | 100 ± 0.0 | |
| FcγRII | 98.0 ± 1.3 | 99.2 ± 0.6 | |
| CD43 | 83.4 ± 7.9 | 93.7 ± 2.0 | |
| MAC-1 | 93.7 ± 1.5 | 94.0 ± 6.7 | |
| CD94 | 43.2 ± 1.5 | 57.4 ± 6.7 | |
| NKG2A/C/E | 41.8 ± 0.9 | 49.2 ± 3.4 | |
| NKG2D | 100 ± 0.0 | 100 ± 0.0 | |
| 2B4 | 96.4 ± 1.1 | 61.4 ± 2.8a | |
| NK1.1 | 100 ± 0.0 | 100 ± 0.0 | |
Marker . | Surface expression in vivo . | . | |
|---|---|---|---|
| . | CD45+/+ . | CD45−/− . | |
| % positive | |||
| CD122 | 90.4 ± 0.9 | 88.4 ± 1.4 | |
| Ly49A | 13.0 ± 0.5 | 11.6 ± 0.8 | |
| Ly49C/I | 42.2 ± 6.5 | 37.2 ± 1.2 | |
| Ly49D | 45.0 ± 3.0 | 32.4 ± 4.3a | |
| Ly49G2 | 39.4 ± 1.1 | 39.4 ± 2.7 | |
| Ly49H | 47.6 ± 2.8 | 44.6 ± 3.3 | |
| Ly49F | 98.4 ± 0.8 | 10.0 ± 1.3 | |
| CD49b | 100 ± 0.0 | 100 ± 0.0 | |
| FcγRII | 98.0 ± 1.3 | 99.2 ± 0.6 | |
| CD43 | 83.4 ± 7.9 | 93.7 ± 2.0 | |
| MAC-1 | 93.7 ± 1.5 | 94.0 ± 6.7 | |
| CD94 | 43.2 ± 1.5 | 57.4 ± 6.7 | |
| NKG2A/C/E | 41.8 ± 0.9 | 49.2 ± 3.4 | |
| NKG2D | 100 ± 0.0 | 100 ± 0.0 | |
| 2B4 | 96.4 ± 1.1 | 61.4 ± 2.8a | |
| NK1.1 | 100 ± 0.0 | 100 ± 0.0 | |
P < 0.045 by Student's t test.
NK1.1+DX5+ splenocytes from CD45+/+ and CD45−/− mice analyzed for the expression of surface markers. Values indicate the mean ± SEM percentages of NK cells deemed positive by flow cytometry from six mice per group.