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Table I.

Amino Acid Sequence of the 22 Overlapping Peptides


NH2-MAEMKTDAATLAQEAGNFERISGDLKTQIDQ-VESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQK-QELDEISTNIRQAGVQYSRADEEQQQALSSQMGF-COOHa
p1b aa 1–15c MAEMKTDAATLAQEA (15)d 
p2 aa 6–20 TDAATLAQEAGNFER (15) 
p3 aa 11–25 LAQEAGNFERISGDL (15) 
p4 aa 16–30 GNFERISGDLKTQI (14) 
p5 aa 20–32 RISGDLKTQIDQV (13) 
p6 aa 23–36 GDLKTQIDQVESTA (14) 
p7 aa 27–39 TQIDQVESTAGSL (13) 
p8 aa 30–44 DQVESTAGSLQGQWR (15) 
p9 aa 35–47 TAGSLQGQWRGAA (13) 
p10 aa 38–51 SLQGQWRGAAGTAA (14) 
p11 aa 41–55 GQWRGAAGTAAQAAV (15) 
p12 aa 46–58 AAGTAAQAAVVRF (13) 
p13 aa 49–62 TAAQAAVVRFQEAA (14) 
p14 aa 53–66 AAVVRFQEAANKQK (14) 
p15 aa 57–69 RFQEAANKQKQEL (13) 
p16 aa 60–72 EAANKQKQELDEI (13) 
p17 aa 63–77 NKQKQELDEISTNIR (15) 
p18 aa 68–81 ELDEISTNIRQAGV (14) 
p19 aa 72–86 ISTNIRQAGVQYSRA (15) 
p20 aa 77–93 RQAGVQYSRADEEQQQA (17) 
p21 aa 84–98 SRADEEQQQALSSQM (15) 
p22
 
aa 89–100
 
EQQQALSSQMGF (12)
 

NH2-MAEMKTDAATLAQEAGNFERISGDLKTQIDQ-VESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQK-QELDEISTNIRQAGVQYSRADEEQQQALSSQMGF-COOHa
p1b aa 1–15c MAEMKTDAATLAQEA (15)d 
p2 aa 6–20 TDAATLAQEAGNFER (15) 
p3 aa 11–25 LAQEAGNFERISGDL (15) 
p4 aa 16–30 GNFERISGDLKTQI (14) 
p5 aa 20–32 RISGDLKTQIDQV (13) 
p6 aa 23–36 GDLKTQIDQVESTA (14) 
p7 aa 27–39 TQIDQVESTAGSL (13) 
p8 aa 30–44 DQVESTAGSLQGQWR (15) 
p9 aa 35–47 TAGSLQGQWRGAA (13) 
p10 aa 38–51 SLQGQWRGAAGTAA (14) 
p11 aa 41–55 GQWRGAAGTAAQAAV (15) 
p12 aa 46–58 AAGTAAQAAVVRF (13) 
p13 aa 49–62 TAAQAAVVRFQEAA (14) 
p14 aa 53–66 AAVVRFQEAANKQK (14) 
p15 aa 57–69 RFQEAANKQKQEL (13) 
p16 aa 60–72 EAANKQKQELDEI (13) 
p17 aa 63–77 NKQKQELDEISTNIR (15) 
p18 aa 68–81 ELDEISTNIRQAGV (14) 
p19 aa 72–86 ISTNIRQAGVQYSRA (15) 
p20 aa 77–93 RQAGVQYSRADEEQQQA (17) 
p21 aa 84–98 SRADEEQQQALSSQM (15) 
p22
 
aa 89–100
 
EQQQALSSQMGF (12)
 
a

Amino acid sequence of the CFP10 protein from M. tuberculosis (reference 37).

b

Peptide name used in the text and in Fig. 1.

c

Location of peptide within the CFP10 protein.

d

Amino acid sequence of peptide and, in parentheses, length.

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