Thymus Cell Counts, Percentages, and Absolute Cell Numbers of CD4+CD8+icTCRβ+ or CD4+CD8+icTCRβ− Cells in Wild Type, Ptenflox/floxLck-Cre, CD3γ−/−, and Ptenflox/floxLck-Cre × CD3γ−/− Mice
Age . | Genotype . | n . | Thymus cellularity . | CD4+CD8+ total . | CD4+CD8+ icTCRβ+ . | CD4+CD8+ icTCRβ+ . | CD4+CD8+ icTCRβ− . |
|---|---|---|---|---|---|---|---|
| wk | no. × 10−6 | % | % | no. × 10−6 | no. × 10−6 | ||
| 1 | wild type | ND | ND | ND | ND | ND | ND |
| Pten−/− | ND | ND | ND | ND | ND | ND | |
| CD3γ−/− | 9 | 4.4 ± 1.4 | 12 ± 4.4 | 88 ± 2.7 | 0.48 ± 0.26 | 0.07 ± 0.04 | |
| Pten−/− × CD3γ−/− | 1 | 3.4 | 26 | 46 | 0.40 | 0.47 | |
| 2 | wild type | 7 | 96 ± 20 | 81 ± 5.6 | 99 ± 0.1 | 80 ± 15 | 0.3 ± 0.1 |
| Pten−/− | 4 | 108 ± 10 | 81 ± 6.8 | 99 ± 0.1 | 88 ± 13 | 0.3 ± 0.1 | |
| CD3γ−/− | 7 | 12 ± 2.4 | 8.7 ± 7.7 | 58 ± 5.1 | 0.64 ± 0.56 | 0.44 ± 0.41 | |
| Pten−/− × CD3γ−/− | 3 | 56 ± 23 | 83 ± 1.5 | 27 ± 9.1 | 13 ± 7.1 | 34 ± 16 | |
| 4 | wild type | 3 | 144 ± 32 | 81 ± 0.9 | 99 ± 0.9 | 114 ± 23 | 2.0 ± 1.2 |
| Pten−/− | 2 | 162 ± 29 | 78 ± 3.3 | 99 ± 0.4 | 124 ± 27 | 1.6 ± 0.8 | |
| CD3γ−/− | 4 | 5.4 ± 0.3 | 2.0 ± 0.7 | 70 ± 7.0 | 0.08 ± 0.03 | 0.03 ± 0.003 | |
| Pten−/− × CD3γ−/− | 3 | 180 ± 4.0 | 72 ± 5.0 | 23 ± 3.0 | 30 ± 5.3 | 100 ± 0.9 |
Age . | Genotype . | n . | Thymus cellularity . | CD4+CD8+ total . | CD4+CD8+ icTCRβ+ . | CD4+CD8+ icTCRβ+ . | CD4+CD8+ icTCRβ− . |
|---|---|---|---|---|---|---|---|
| wk | no. × 10−6 | % | % | no. × 10−6 | no. × 10−6 | ||
| 1 | wild type | ND | ND | ND | ND | ND | ND |
| Pten−/− | ND | ND | ND | ND | ND | ND | |
| CD3γ−/− | 9 | 4.4 ± 1.4 | 12 ± 4.4 | 88 ± 2.7 | 0.48 ± 0.26 | 0.07 ± 0.04 | |
| Pten−/− × CD3γ−/− | 1 | 3.4 | 26 | 46 | 0.40 | 0.47 | |
| 2 | wild type | 7 | 96 ± 20 | 81 ± 5.6 | 99 ± 0.1 | 80 ± 15 | 0.3 ± 0.1 |
| Pten−/− | 4 | 108 ± 10 | 81 ± 6.8 | 99 ± 0.1 | 88 ± 13 | 0.3 ± 0.1 | |
| CD3γ−/− | 7 | 12 ± 2.4 | 8.7 ± 7.7 | 58 ± 5.1 | 0.64 ± 0.56 | 0.44 ± 0.41 | |
| Pten−/− × CD3γ−/− | 3 | 56 ± 23 | 83 ± 1.5 | 27 ± 9.1 | 13 ± 7.1 | 34 ± 16 | |
| 4 | wild type | 3 | 144 ± 32 | 81 ± 0.9 | 99 ± 0.9 | 114 ± 23 | 2.0 ± 1.2 |
| Pten−/− | 2 | 162 ± 29 | 78 ± 3.3 | 99 ± 0.4 | 124 ± 27 | 1.6 ± 0.8 | |
| CD3γ−/− | 4 | 5.4 ± 0.3 | 2.0 ± 0.7 | 70 ± 7.0 | 0.08 ± 0.03 | 0.03 ± 0.003 | |
| Pten−/− × CD3γ−/− | 3 | 180 ± 4.0 | 72 ± 5.0 | 23 ± 3.0 | 30 ± 5.3 | 100 ± 0.9 |