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Table I.

Induction of IL-10 in T Cells and Macrophages by TGF-β1–expressing Constructs




TGF-β 1 (pg/ml)b

IL-10 (pg/ml)
Cell linea
Plasmid/retrovirus
mock
TGF-β
mock
TGF-β
T cell      
OVA-3 T cell clone regional LN <30 327 <25 1,620 
EL-4 (anti-CD3/anti-CD28) thymus 184 353 <25  <25 
Macrophage      
RAW264 (LPS 0.5 μg) Mθ 214 924 181 930 
MH-S (LPS 0.5 μg) alveolar Mθ 119 636 <25 161 
Epithelial      
CMT93 colon epithelial 440 1,270 <25  <25 
C10 lung epithelial <30 468 44  <25 
E10 lung epithelial <30 1,028 <25  <25 
LA-4 lung epithelial Ca <30 128 <25  <25 
KLN205 lung squamous 88 552 48  <25 
Fibroblast      
MLg lung fibroblast <30 268 <25  <25 
3T3
 
embryo fibroblast
 
128
 
1,430
 
<25
 
<25
 



TGF-β 1 (pg/ml)b

IL-10 (pg/ml)
Cell linea
Plasmid/retrovirus
mock
TGF-β
mock
TGF-β
T cell      
OVA-3 T cell clone regional LN <30 327 <25 1,620 
EL-4 (anti-CD3/anti-CD28) thymus 184 353 <25  <25 
Macrophage      
RAW264 (LPS 0.5 μg) Mθ 214 924 181 930 
MH-S (LPS 0.5 μg) alveolar Mθ 119 636 <25 161 
Epithelial      
CMT93 colon epithelial 440 1,270 <25  <25 
C10 lung epithelial <30 468 44  <25 
E10 lung epithelial <30 1,028 <25  <25 
LA-4 lung epithelial Ca <30 128 <25  <25 
KLN205 lung squamous 88 552 48  <25 
Fibroblast      
MLg lung fibroblast <30 268 <25  <25 
3T3
 
embryo fibroblast
 
128
 
1,430
 
<25
 
<25
 
a

Mouse cell lines were transfected with pCI-TGF-β1 plasmid using TransfectAMINE 2000 or by electroporation (EL-4). The OVA-3 T cell clone was infected with a retrovirus of pBMN-TGF-β1-GFP.

b

TGF-β1 ELISA was performed after acid treatment.

The value obtained for each cell line is the mean value from three independent experiments.

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