Protease Activities in Peritoneal Cells
Source of cells . | Chymotrypsin-like activity mOD/(min × 106 cells) . | Trypsin-like activity mOD/(min × 106 cells) . | CPA activity mOD/(min × 106 cells) . |
|---|---|---|---|
| mMCP-4+/+ mice | 3.5 ± 2.9 | 2.0 ± 0.077 | −14.3 ± 5.0 |
| (n = 4) | (n = 4) | (n = 4) | |
| mMCP-4−/− mice | n.d. | 3.5 ± 2.0 | −14.7 ± 4.67 |
| (n = 4) | (n = 4) | (n = 4) | |
| mMCP-4+/− mice | 2.2 ± 1.58 | 1.9 ± 0.51 | −15.6 ± 3.07 |
| (n = 3) | (n = 4) | (n = 3) |
Source of cells . | Chymotrypsin-like activity mOD/(min × 106 cells) . | Trypsin-like activity mOD/(min × 106 cells) . | CPA activity mOD/(min × 106 cells) . |
|---|---|---|---|
| mMCP-4+/+ mice | 3.5 ± 2.9 | 2.0 ± 0.077 | −14.3 ± 5.0 |
| (n = 4) | (n = 4) | (n = 4) | |
| mMCP-4−/− mice | n.d. | 3.5 ± 2.0 | −14.7 ± 4.67 |
| (n = 4) | (n = 4) | (n = 4) | |
| mMCP-4+/− mice | 2.2 ± 1.58 | 1.9 ± 0.51 | −15.6 ± 3.07 |
| (n = 3) | (n = 4) | (n = 3) |
Extracts were prepared from peritoneal cells and were assayed for trypsin-like, chymotrypsin-like, and CPA activities as described in Materials and Methods. Note that hydrolysis of the CPA substrate results in decreased absorbance. n.d., none detected.