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Table I.

Host Cell–free Transformation into Hepatic Stages


Conditionsa

Spherical
 trophozoitesb

Transformationc
°C  
37 6,725.34 13.43 
22 34.67 0.07 
22 → 37 9,656 19.32 
37 w/o FBS
 
346.67
 
0.69
 

Conditionsa

Spherical
 trophozoitesb

Transformationc
°C  
37 6,725.34 13.43 
22 34.67 0.07 
22 → 37 9,656 19.32 
37 w/o FBS
 
346.67
 
0.69
 
a

24-h cultures, except 22 → 37°C, shift is 48 h; (Materials and Methods for culture conditions).

b

Average number from three independent experiments. Parasites were scored as completely transformed when they appeared spherical with no sporozoite remnants visible. (Fig. 1 D).

c

Percentage of transformation was calculated based on the 5 × 104 sporozoites used for culture.

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