Skip to Main Content
Table I.

Administration of Anti–IL-10 mAb Blocks Exacerbation of Anemia in Fas-deficient H+L6 Homozygous Mice


Treatments

Hematocrit
 values (%)

Amounts of the autoAb
 bound to RBCs
 (mean fluorescence
 intensity)

Ig-producing
 cells
 (per 105 cells)
Anti–IL-10 37.5 ± 2.5a 494.4 ± 364.2b 235.8 ± 173.1b 
Control Ig
 
31.5 ± 0.5
 
1,419.2 ± 497.2
 
660.6 ± 240.2
 

Treatments

Hematocrit
 values (%)

Amounts of the autoAb
 bound to RBCs
 (mean fluorescence
 intensity)

Ig-producing
 cells
 (per 105 cells)
Anti–IL-10 37.5 ± 2.5a 494.4 ± 364.2b 235.8 ± 173.1b 
Control Ig
 
31.5 ± 0.5
 
1,419.2 ± 497.2
 
660.6 ± 240.2
 
a

P < 0.01.

b

P < 0.02.

Either anti–IL-10 mAb or control rat IgG (100 μg/injection) was injected weekly into Fas-deficient H+L6 homozygous mice. 4 wk after the first injection, hematocrit values (%) were measured. Amounts of the autoAb bound to RBCs were assessed as mean fluorescence intensity of FITC-conjugated anti-IgM Ab bound to RBCs. Numbers of anti-RBC Ab-producing cells in MLN were measured by ELISPOT assay using anti-IgM Ab. Values represent the mean ± SD of five mice in each experimental group. Paired t test values calculated between anti–IL-10 and control rat IgG.

Close Modal

or Create an Account

Close Modal
Close Modal