Validation of the Deamidation Patterns
. | Pattern . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Peptides . | QP ↓ . | QXXF ↑ . | QXXP ↓ . | QXP ↑ . | |||
| 1 VPVPQLQPQNPSQQQPQEQVPL | 100% | 100% | 100% | 66% | |||
| 2 PLVQQQQFLGQQQPFPPQ | 100% | 100% | 100% | 100% | |||
| 3 YYPTSPQQSGQG | - | - | - | - | |||
| 4 QGQQGYYPTSPQ | - | 50% | - | - | |||
| 5 SSQVSFQPSQLN | 100% | 100% | - | - | |||
| 6 FPQTQQPQQLFPQSQ | 100% | 0% | 100% | 100% | |||
| 7 GQQGYYPTSVQQSGQ | - | 50% | - | - | |||
| 8 GSVQPQQQLPQFEIR | 100% | 100% | 100% | 100% | |||
| 9 FPQHCNYQQQPQTFPQPF | 100% | - | 100% | 100% | |||
| 10 QQPIQPQQFPQQQFF | 100% | 50% | 100% | 50% | |||
| 11 QSGQYQQQPQMQTT | 100% | - | 100% | 100% | |||
| 12 ESQQSQQDEPQPF | 100% | - | 100% | - | |||
| 13 FPQPEDQQSQQSE | 100% | - | - | - | |||
| 14 LQQVQQGPQQQPFPQPQPF | 100% | 100% | 66% | 100% | |||
| 15 QVQWPQQQPFPQPQQPF | 100% | 50% | 100% | 33% | |||
| 16 PLLPQQPFPSQQEQPQF | 100% | 50%a | 100% | 100% | |||
| 17 WQQQPPFSQEQPIL | 100% | 0%a | 33% | 100% | |||
| 18 QSNLPQPAQQPFQPQVPQQP | 100% | 100% | - | 66% | |||
| 19 WFQPSQLNPQAQQDQPQ | 100% | - | 100% | - | |||
| 20 LQQPQQPQFQPQQQF | 100% | 50%a | 100% | 50% | |||
| 21 VQQQIPVVQPSIL | 100% | 33%a | 100% | 100% | |||
. | Pattern . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Peptides . | QP ↓ . | QXXF ↑ . | QXXP ↓ . | QXP ↑ . | |||
| 1 VPVPQLQPQNPSQQQPQEQVPL | 100% | 100% | 100% | 66% | |||
| 2 PLVQQQQFLGQQQPFPPQ | 100% | 100% | 100% | 100% | |||
| 3 YYPTSPQQSGQG | - | - | - | - | |||
| 4 QGQQGYYPTSPQ | - | 50% | - | - | |||
| 5 SSQVSFQPSQLN | 100% | 100% | - | - | |||
| 6 FPQTQQPQQLFPQSQ | 100% | 0% | 100% | 100% | |||
| 7 GQQGYYPTSVQQSGQ | - | 50% | - | - | |||
| 8 GSVQPQQQLPQFEIR | 100% | 100% | 100% | 100% | |||
| 9 FPQHCNYQQQPQTFPQPF | 100% | - | 100% | 100% | |||
| 10 QQPIQPQQFPQQQFF | 100% | 50% | 100% | 50% | |||
| 11 QSGQYQQQPQMQTT | 100% | - | 100% | 100% | |||
| 12 ESQQSQQDEPQPF | 100% | - | 100% | - | |||
| 13 FPQPEDQQSQQSE | 100% | - | - | - | |||
| 14 LQQVQQGPQQQPFPQPQPF | 100% | 100% | 66% | 100% | |||
| 15 QVQWPQQQPFPQPQQPF | 100% | 50% | 100% | 33% | |||
| 16 PLLPQQPFPSQQEQPQF | 100% | 50%a | 100% | 100% | |||
| 17 WQQQPPFSQEQPIL | 100% | 0%a | 33% | 100% | |||
| 18 QSNLPQPAQQPFQPQVPQQP | 100% | 100% | - | 66% | |||
| 19 WFQPSQLNPQAQQDQPQ | 100% | - | 100% | - | |||
| 20 LQQPQQPQFQPQQQF | 100% | 50%a | 100% | 50% | |||
| 21 VQQQIPVVQPSIL | 100% | 33%a | 100% | 100% | |||
QP inhibition of deamidation overrules the positive influence of QXXF.
The observed deamidation patterns in a set of 21 gluten peptides were compared with those predicted according to the deamidation patterns given in Fig. 1. The deamidation patterns are represented in the 4 columns. The percentages shown indicate whether the pattern is fully or partially applicable to the specific deamidation of Q residues in the listed gluten peptides. A bar (-) indicates the absence of that sequence in the peptide. Deamidated glutamine residues are shown in bold and underlined.