Cytokine Production by CD14 Tetramer-stained T Cell Subsets
Stimulation . | Tetramer subset . | IFN-γ . | TNF-α . | GM-CSF . | IL-2 . | IL-4 . | IL-5 . | IL-6 . | IL-10 . | IL-13 . |
|---|---|---|---|---|---|---|---|---|---|---|
| None | CD4+ | 2.7 (1.5) | 2.3 (1.9) | 1.7 (1.2) | 1.7 (1.2) | 3.2 (2.5) | 0.9 (1.0) | 3.1 (1.1) | 1.6 (1.6) | 2.6 (2.9) |
| CD4− | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0.7 (1.5) | 0 (0) | 0 (0) | 0.3 (0.6) | 0 (0) | |
| PMA/iono | CD4+ | 92 (1.5) | 87 (4.6) | 48 (23) | 54 (22) | 36 (11) | 19 (5.4) | 23 (10) | 22 (2.5) | 22 (5.7) |
| CD4− | 91 (7.1) | 72 (5.9) | 18 (16) | 11 (5.6) | 9.4 (5.8) | 0 (0) | 0.6 (1.1) | 0.3 (0.6) | 0.3 (0.6) | |
| α-GalCer | CD4+ | 45 (8.6) | 32 (3.5) | 12 (15) | 13 (13) | 16 (9.5) | 10 (7.5) | 9 (6.4) | 10 (11) | 12 (13) |
| CD4− | 35 (19) | 25 (14) | 0 (0) | 0.7 (1.4) | 3.1 (.9) | 0 (0) | 2.9 (3.8) | 1.1 (2.3) | 0 (0) | |
| PMA/iono | Negative | 22 (13) | 17 (11) | 2.6 (1.8) | 20 (8.9) | 1.0 (0.8) | 0.08 (0.11) | 0.02 (0.03) | 0.5 (0.4) | 0.6 (0.3) |
Stimulation . | Tetramer subset . | IFN-γ . | TNF-α . | GM-CSF . | IL-2 . | IL-4 . | IL-5 . | IL-6 . | IL-10 . | IL-13 . |
|---|---|---|---|---|---|---|---|---|---|---|
| None | CD4+ | 2.7 (1.5) | 2.3 (1.9) | 1.7 (1.2) | 1.7 (1.2) | 3.2 (2.5) | 0.9 (1.0) | 3.1 (1.1) | 1.6 (1.6) | 2.6 (2.9) |
| CD4− | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0.7 (1.5) | 0 (0) | 0 (0) | 0.3 (0.6) | 0 (0) | |
| PMA/iono | CD4+ | 92 (1.5) | 87 (4.6) | 48 (23) | 54 (22) | 36 (11) | 19 (5.4) | 23 (10) | 22 (2.5) | 22 (5.7) |
| CD4− | 91 (7.1) | 72 (5.9) | 18 (16) | 11 (5.6) | 9.4 (5.8) | 0 (0) | 0.6 (1.1) | 0.3 (0.6) | 0.3 (0.6) | |
| α-GalCer | CD4+ | 45 (8.6) | 32 (3.5) | 12 (15) | 13 (13) | 16 (9.5) | 10 (7.5) | 9 (6.4) | 10 (11) | 12 (13) |
| CD4− | 35 (19) | 25 (14) | 0 (0) | 0.7 (1.4) | 3.1 (.9) | 0 (0) | 2.9 (3.8) | 1.1 (2.3) | 0 (0) | |
| PMA/iono | Negative | 22 (13) | 17 (11) | 2.6 (1.8) | 20 (8.9) | 1.0 (0.8) | 0.08 (0.11) | 0.02 (0.03) | 0.5 (0.4) | 0.6 (0.3) |
The percentage of CD4+ and CD4− CD1d tetramer positive lymphocytes that produced intracellular cytokines after α-GalCer, PMA/ionomycin, or no stimulation. For comparison, the percentage of CD1d tetramer negative T cells that produced intracellular cytokines after PMA/ionomycin stimulation is also shown. The mean percentages of cells that stained positively are given first, with the standard deviations shown in parentheses. The results are compiled from 2–4 independent analyses of PBMC samples from three to six healthy donors.