Lymphocyte Populations in mkk7−/− Chimeric Mice
| Subsets . | mkk7+/− →rag1−/− . | mkk7−/− →rag1−/− . |
|---|---|---|
| Thymus | ||
| Total cell number (×107) | 9.84 ± 2.2 | 22.5 ± 4.6 |
| CD4+CD8+ (%) | 81.2 ± 7.6 | 88.9 ± 2.3 |
| CD4+CD8+ (%) | 11.8 ± 5.0 | 7.60 ± 1.2 |
| CD4+CD8+ (%) | 2.5 ± 0.8 | 1.2 ± 0.4 |
| Spleen | ||
| Total cell number (×107) | 2.90 ± 0.6 | 2.74 ± 0.4 |
| TCRα/β+ (%) | 36.3 ± 4.3 | 33.8 ± 4.9 |
| B220+sIgM+ (%) | 29.9 ± 5.4 | 27.8 ± 5.6 |
| Lymph node | ||
| Total cell number (×107) | 2.96 ± 0.4 | 3.14 ± 0.3 |
| CD4+CD8− (%) | 53.2 ± 4.0 | 43.6 ± 2.6 |
| CD4−CD8+ (%) | 19.7 ± 1.6 | 15.5 ± 2.8 |
| Subsets . | mkk7+/− →rag1−/− . | mkk7−/− →rag1−/− . |
|---|---|---|
| Thymus | ||
| Total cell number (×107) | 9.84 ± 2.2 | 22.5 ± 4.6 |
| CD4+CD8+ (%) | 81.2 ± 7.6 | 88.9 ± 2.3 |
| CD4+CD8+ (%) | 11.8 ± 5.0 | 7.60 ± 1.2 |
| CD4+CD8+ (%) | 2.5 ± 0.8 | 1.2 ± 0.4 |
| Spleen | ||
| Total cell number (×107) | 2.90 ± 0.6 | 2.74 ± 0.4 |
| TCRα/β+ (%) | 36.3 ± 4.3 | 33.8 ± 4.9 |
| B220+sIgM+ (%) | 29.9 ± 5.4 | 27.8 ± 5.6 |
| Lymph node | ||
| Total cell number (×107) | 2.96 ± 0.4 | 3.14 ± 0.3 |
| CD4+CD8− (%) | 53.2 ± 4.0 | 43.6 ± 2.6 |
| CD4−CD8+ (%) | 19.7 ± 1.6 | 15.5 ± 2.8 |
7–12-wk-old mkk7+/−→rag1−/− and mkk7−/−→rag1−/− mice were used. Total cells from thymi (n = 8), spleen (n = 5), and lymph nodes (n = 5) were stained with Abs against the indicated proteins and populations determined by FACScan™. Bold numbers indicate statistically significant differences between mkk7+/− and mkk7−/− chimeric mice (Student's t test; P < 0.05). Values are given as the mean ± S.E.M.