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Table 1
Days after immunizationPrecursor frequencyNumber of LN cellsTotal number of SWM-specific Vβ8+ cells
  1/x z × 106 (z/100)/x 
Primary 1/3,150 10.23 33 
 1/38 12.8 3,368 
 1/65 9.44 1,452 
 14 1/362 15.6 431 
 28 1/405 8.73 215 
Secondary 1/217 23.55 1,085 
 1/37 20.95 5,662 
 1/42 20.00 4,762 
 1/76 12.65 1,665 
 14 1/125 10.35 828 
Days after immunizationPrecursor frequencyNumber of LN cellsTotal number of SWM-specific Vβ8+ cells
  1/x z × 106 (z/100)/x 
Primary 1/3,150 10.23 33 
 1/38 12.8 3,368 
 1/65 9.44 1,452 
 14 1/362 15.6 431 
 28 1/405 8.73 215 
Secondary 1/217 23.55 1,085 
 1/37 20.95 5,662 
 1/42 20.00 4,762 
 1/76 12.65 1,665 
 14 1/125 10.35 828 

Determination by LDA of antigen-specific precursor frequencies in the CD4highVβ8+ population. The total number of antigen-specific precursor cells present in the LNs during the primary and secondary response to SWM 110–112 in the Vβ8+ population was derived from the precursor frequency of antigen-specific T cells in the CD4highVβ8+ subset and from the total number of LN cells (z), using the equation n = (z/100)/x. CD4highVβ8+ cells were sorted from primed LNs at days 3, 6, 8, 14, and 28 after primary immunization and days 2, 4, 6, 8, and 14 after secondary immunization. LDA was performed and the precursor frequency (1/x) was derived by Poisson statistics as described in Materials and Methods. Shown are the results of one representative LDA experiment performed at each selected time point.

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