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Table 1

Relative DO/DM Expression Levels in Mel JuSO/DOαβGFP Transfectants and Primary B Cells

ActinDMαDOβDOβ/DMα
 aU aU aU aU 
Mel JuSo/DOαβGFP     
Population 2 1.2 0.04 0.03 
Population 3 1.1 0.07 0.06 
Population 4 1.1 0.9 0.82 
Population 5 
Primary B Cells 1.0–1.4 0.45 0.3–0.5 
ActinDMαDOβDOβ/DMα
 aU aU aU aU 
Mel JuSo/DOαβGFP     
Population 2 1.2 0.04 0.03 
Population 3 1.1 0.07 0.06 
Population 4 1.1 0.9 0.82 
Population 5 
Primary B Cells 1.0–1.4 0.45 0.3–0.5 

Quantification of DMα and DOβ expression levels in transfectant Mel JuSo cells sorted for different expression levels of DOαβGFP and primary B cells was performed using the FluorChem™ imaging system and AlphaEase™ FC software. Relative DMα and DOβ expression levels (in arbitrary units, aU) are correlated to cellular actin levels (in arbitrary units).

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