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Table 2

Ability of Chimeric Receptors to Support the Outgrowth of Cells Expressing a μ, λ1 Antigen Receptor

ConstructnOutgrowth of μ+, λ+ cells
λ1–IRES–huIL-4R/IL-7R − 
λ1–IRES–huIL-4R/IL-2Rβ 
λ1–IRES–huIL-4R/IL-7RY410F 
ConstructnOutgrowth of μ+, λ+ cells
λ1–IRES–huIL-4R/IL-7R − 
λ1–IRES–huIL-4R/IL-2Rβ 
λ1–IRES–huIL-4R/IL-7RY410F 

Pre-B cells were transduced with bicistronic retroviruses encoding a λ1 light chain cDNA linked through an IRES to chimeric cytokine receptors. Transduced cells were selected in human IL-4 (10 ng/ml) for 7 d, and the surface expression of μ and λ was determined by flow cytometry (see Materials and Methods). n, the number of independent experiments.

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