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Table 1

κ+ Cells Responsive to IL-7 Cannot Be Cloned Out

Cell dilutionNo. of clones analyzedNo. of κ clones (≤5% κ+)No. of κ+ clones
0.3 cells/well 100 100 
Cell dilutionNo. of clones analyzedNo. of κ clones (≤5% κ+)No. of κ+ clones
0.3 cells/well 100 100 

Pre-B cells were cloned at limiting dilution (0.3 cells/well) in 96-well plates using culture medium containing 10 ng/ml of recombinant IL-7. Cell surface expression of μ and κ in individual clones was determined by flow cytometry as described above.

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