Recognition of the α-gliadin recombinants is compared with their sequences. Negative responses (−) all had a stimulation index of 1.6 or less, whereas positive responses (+) were greater than or equal to 5. The shaded region in the α-9 recombinant marks a peptide with a suitable mass to that obtained by MS analysis and is present in all gliadins that stimulated the clone. The shaded region in α-2 marks a related but unique sequence present only in this recombinant.