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Table 2

Effect of Increased Cytosolic Ca2+ on Intracellular Growth of M. tuberculosis

M. tuberculosis CFUs
24 h48 h
DonorControlA23187ControlA23187
77,250 39,375 297,735 191,250 
365,625 208,875 867,735 563,250 
330,375 177,375 912,375 568,875 
265,500 95,250 217,500 58,500 
153,000 80,500 254,125 98,750 
M. tuberculosis CFUs
24 h48 h
DonorControlA23187ControlA23187
77,250 39,375 297,735 191,250 
365,625 208,875 867,735 563,250 
330,375 177,375 912,375 568,875 
265,500 95,250 217,500 58,500 
153,000 80,500 254,125 98,750 

Macrophages were infected with C-op Erdman M. tuberculosis at an MOI of 1:1 in the presence or absence of 1 μM A23187 in CHBSS. 20 min later, MDMs were washed and repleted with fresh media containing 2.5% autologous serum and 20 μg/ml phosphatidylcholine vesicles. 24 and 48 h after infection, detergent lysates of infected macrophages were plated on 7H11 plates, and the viability of intracellular M. tuberculosis was quantitated by determination of CFUs.

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