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Table 1

Opsonization of M. tuberculosis with Polyclonal Abs to LAM Results in Decreased Survival within Human Macrophages

DonorM. tuberculosis CFUs
1 h48 h96 h
C-opAb-opC-opAb-opC-opAb-op
36,000 19,500 406,500 73,500 531,750 192,750 
4,125 2,250 19,500 9,375 203,625 33,750 
13,688 8,250 403,875 68,625 ND ND 
10,125 6,938 123,000 45,375 840,375 181,500 
7,500 4,313 187,125 25,500 841,125 182,625 
43,688 18,188 383,250 80,625 784,875 110,625 
DonorM. tuberculosis CFUs
1 h48 h96 h
C-opAb-opC-opAb-opC-opAb-op
36,000 19,500 406,500 73,500 531,750 192,750 
4,125 2,250 19,500 9,375 203,625 33,750 
13,688 8,250 403,875 68,625 ND ND 
10,125 6,938 123,000 45,375 840,375 181,500 
7,500 4,313 187,125 25,500 841,125 182,625 
43,688 18,188 383,250 80,625 784,875 110,625 

Erdman M. tuberculosis was opsonized with autologous human serum (C-op) or 5 μg/ml of rabbit polyclonal Ab to LAM (Ab-op). After extensive washing of the bacilli, MDMs were infected at an MOI of 1:1. 1 h after infection, monolayers were washed and repleted with fresh media. Detergent lysates were prepared from infected MDMs (monolayers plus supernatants) 1, 48, and 96 h after infection, and viability of intracellular M. tuberculosis was analyzed by determination of CFUs on 7H11 plates as described in Materials and Methods.

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