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Table III

Inhibition of Lymph Node Cell Proliferation to MBPp85–99 by Microbial Mimicry Peptides

PeptideSequenceRelative affinity* for I-AsInhibition of LNC responses
      % 
MBPp85–99  ENPVVHFFKNIVTPR  667*  – 
HPV 7  IGGRVHFFKDISPIA  208  62 
HPV 40  IGGRVHFFRDISPIG  180  39 
Herpes simplex DNA pol  GGRRLFFVKAHVRE  551  39 
Herpes simplex VP16  DMTPADALDDRDLEM  ND  29 
PeptideSequenceRelative affinity* for I-AsInhibition of LNC responses
      % 
MBPp85–99  ENPVVHFFKNIVTPR  667*  – 
HPV 7  IGGRVHFFKDISPIA  208  62 
HPV 40  IGGRVHFFRDISPIG  180  39 
Herpes simplex DNA pol  GGRRLFFVKAHVRE  551  39 
Herpes simplex VP16  DMTPADALDDRDLEM  ND  29 
*

 Peptide binding assays on purified I-As molecules were performed as described in reference 22. Results are expressed as IC50 in nanomolars. E. coli E7 peptide bound at an IC50 of 76 nM.  

 For inhibition of lymph node cell (LNC) proliferative responses by microbial mimicry peptides, LNCs from (PLSJL/J)F1 mice immunized with the MBPp85–99 peptide were incubated in vitro in the presence of both the MBPp85–99 peptide and a molecular mimicry peptide at a molar ratio of 1:1 (final concentration of each peptide was 0.01 mg/ml). CPM incorporation of the LNCs incubated with MBPp85–99 at 0.01 mg/ml were 5,792 in the absence of inhibitor and CPM of LNCs incubated with medium alone was 1,010. Percentage of inhibition was calculated with the formula: % of inhibition = (1 − SI with inhibitor / SI without inhibitor) × 100.  

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