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Table II

Liver Histologic (Granulomatous) Response to L. donovani Infection in X-CGD and iNOS KO Mice

Weeks after infection
X-CGDC57BL/6iNOS KOWT Controls
Tissue reaction248248248248
None (%)  70  10   0  19   0   0  81  32   1  22   0   0 
Granuloma                         
 Developing (%)  29  81  39  78  63   2  19  65  38  57  69   6 
 Mature (%)   1   9  33   3  37  32   0   3  61  21  31  43 
 Empty (%)   0   0  28   0   0  66   0   0   0   0   0  51 
Weeks after infection
X-CGDC57BL/6iNOS KOWT Controls
Tissue reaction248248248248
None (%)  70  10   0  19   0   0  81  32   1  22   0   0 
Granuloma                         
 Developing (%)  29  81  39  78  63   2  19  65  38  57  69   6 
 Mature (%)   1   9  33   3  37  32   0   3  61  21  31  43 
 Empty (%)   0   0  28   0   0  66   0   0   0   0   0  51 

All infected foci in 50 consecutive ×63 microscopic fields were scored as follows for granuloma formation: none, single or fused parasitized Kupffer cells with no mononuclear cell infiltrate; developing, core of fused infected Kupffer cells with some cell infiltrate; mature, fused Kupffer cell core surrounded by a well-developed mononuclear cell mantle; and empty, granuloma devoid of amastigotes (1–4). See Figs. 2 and 3. Results are from two experiments, and indicate mean values for four to five mice at each time point. SEM, omitted for clarity, were all <12%.  

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