Capacity of Defined Precursor Populations from C57BL/6 and Rag-1−/− Mice to Differentiate into Myeloid and Erythroid Lineage Cells
| Mouse . | . | Cells cultured . | . | CFU-GM . | . | CFU-BFE . | . | CFU-GEMM . |
|---|---|---|---|---|---|---|---|---|
| C57BL/6 | Stem | 26.4 (5.0) | 6.80 (1.2) | 8.20 (1.5) | ||||
| A0 | 14.2 (1.4) | 1.70 (0.5) | 0.68 (0.15) | |||||
| A1 | 0 | 0 | 0 | |||||
| A2 | 0 | 0 | 0 | |||||
| Rag-1−/− | A0 | 15.1 (1.17) | 1.58 (0.15) | 0.89 (0.51) | ||||
| A1 | 0 | 0 | 0 | |||||
| A2 | 0 | 0 | 0 |
| Mouse . | . | Cells cultured . | . | CFU-GM . | . | CFU-BFE . | . | CFU-GEMM . |
|---|---|---|---|---|---|---|---|---|
| C57BL/6 | Stem | 26.4 (5.0) | 6.80 (1.2) | 8.20 (1.5) | ||||
| A0 | 14.2 (1.4) | 1.70 (0.5) | 0.68 (0.15) | |||||
| A1 | 0 | 0 | 0 | |||||
| A2 | 0 | 0 | 0 | |||||
| Rag-1−/− | A0 | 15.1 (1.17) | 1.58 (0.15) | 0.89 (0.51) | ||||
| A1 | 0 | 0 | 0 | |||||
| A2 | 0 | 0 | 0 |
2,000 (stem, Lin− Sca-1+) or 4,000 (Frs. A0–A2) sorted cells were cultured in methylcellulose medium optimized for myeloid and erythroid cell types. After 12 d, cultures were scored for the number of granulocytic/macrophage (CFU-GM), erythroid (CFU-BFE), and mixed (CFU-GEMM) colonies. Means for triplicate cultures are shown with SDs in parentheses. Data are normalized for 1,000 input cells/culture.