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Table I

TNF-α–induced Leukocyte Infiltration in Murine Air Pouches: Inhibitory Action of 15R/S-methyl-LXA₄

Injection	Number of leukocytes present in pouch (×10⁶)
Injection	Neutrophils	Eosinophils/ basophils	Monocytes/ macrophages
TNF-α	2.40 ± 0.10^*	0.30 ± 0.01^*	0.20 ± 0.01^*
15R/S-methyl-LXA₄	0.98 ± 0.10^‡	0.13 ± 0.01^‡	0.10 ± 0.01^‡
+ TNF-α	(59.1%)	(56.0%)	(50%)
15R/S-methyl-LXA₄	0.25 ± 0.01	0.03 ± 0.01	0.14 ± 0.01^*
Vehicle	0.30 ± 0.01	0.07 ± 0.01	0.06 ± 0.01

Injection	Number of leukocytes present in pouch (×10⁶)
Injection	Neutrophils	Eosinophils/ basophils	Monocytes/ macrophages
TNF-α	2.40 ± 0.10^*	0.30 ± 0.01^*	0.20 ± 0.01^*
15R/S-methyl-LXA₄	0.98 ± 0.10^‡	0.13 ± 0.01^‡	0.10 ± 0.01^‡
+ TNF-α	(59.1%)	(56.0%)	(50%)
15R/S-methyl-LXA₄	0.25 ± 0.01	0.03 ± 0.01	0.14 ± 0.01^*
Vehicle	0.30 ± 0.01	0.07 ± 0.01	0.06 ± 0.01

Air pouches were raised as described in Materials and Methods. Each mouse was injected with 1 ml PBS containing vehicle (0.1% ethanol), TNF-α (10 ng), 15R/S-methyl-LXA₄ (25 nmol), or TNF-α plus 15R/S-methyl-LXA₄. Leukocyte infiltration was determined 4 h after injection. Results present the mean ± SEM of three different mice. Percent inhibition is indicated in parentheses. Statistically different from

vehicle-injected mice (P < 0.01) and

‡

TNF-α–injected mice (P < 0.01).

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