Analysis of VκJκ Joins in VκJκ-intron-RS Sequences: Models and Predictions Compared with Experimental Data
| Models . | . | Predicted fraction in-frame . | . | Experimental data . | . | Observed percentage in frame . |
|---|---|---|---|---|---|---|
| Perfect feedback regulation with functional κ chain preventing λ rearrangement | 0% | 27 out of 57 VκJκ-intron-RS loci | 47.4% (P < 0.04) | |||
| Poor feedback regulation | ≤33% | |||||
| High frequency of Vκ pseudogenes | ≤33% | |||||
| High frequency of H/κ chain mispairing | ≤33% | |||||
| Any combination of the above | ≤33% | |||||
| Extreme model of editing with random RS rearrangements and all κs autoreactive | 33% |
| Models . | . | Predicted fraction in-frame . | . | Experimental data . | . | Observed percentage in frame . |
|---|---|---|---|---|---|---|
| Perfect feedback regulation with functional κ chain preventing λ rearrangement | 0% | 27 out of 57 VκJκ-intron-RS loci | 47.4% (P < 0.04) | |||
| Poor feedback regulation | ≤33% | |||||
| High frequency of Vκ pseudogenes | ≤33% | |||||
| High frequency of H/κ chain mispairing | ≤33% | |||||
| Any combination of the above | ≤33% | |||||
| Extreme model of editing with random RS rearrangements and all κs autoreactive | 33% |