κ Locus Rearrangement Status of IgMλ Hybridomas
| . | . | . | . | κ locus genotype . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | Hybridomas . | . | RS− . | . | Vκ-RS . | . | VκJκ-RS . | . | Productive/ nonproductive . |
| n | ||||||||||
| Fusion 1 | 37 | 12 | 16 | 9/9* | 5/4 | |||||
| Fusion 2 | 44 | 10 | 30 | 4/4 | 2/2 | |||||
| Fusion 3 | 12 | 1 | 6 | 5/4 | 4/0 | |||||
| Fusion 4 | 25 | 9 | 11 | 5/2 | 1/1 | |||||
| Fusion 5 | 15 | 2 | 10 | 3/1 | 0/1 | |||||
| Totals | 133 | 34 | 73 | 26/20 | 12/8 | |||||
| . | . | . | . | κ locus genotype . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | Hybridomas . | . | RS− . | . | Vκ-RS . | . | VκJκ-RS . | . | Productive/ nonproductive . |
| n | ||||||||||
| Fusion 1 | 37 | 12 | 16 | 9/9* | 5/4 | |||||
| Fusion 2 | 44 | 10 | 30 | 4/4 | 2/2 | |||||
| Fusion 3 | 12 | 1 | 6 | 5/4 | 4/0 | |||||
| Fusion 4 | 25 | 9 | 11 | 5/2 | 1/1 | |||||
| Fusion 5 | 15 | 2 | 10 | 3/1 | 0/1 | |||||
| Totals | 133 | 34 | 73 | 26/20 | 12/8 | |||||
RS− denotes hybridomas that lacked a detectable RS rearrangement. Vκ-RS and VκJκ-RS are defined in Fig. 1. Asterisk indicates total number of VκJκ-intron-RS loci that were isolated followed by the number that we were able to PCR amplify with the consensus FWR3 oligo.