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Table 1

Analyses of Peritoneal Lavage 3 h after CLP

+/+−/−−/− IgM−/− PtC-IgM−/− PC-IgM
TNF-α (pg/ml)  1,507  892  2,068  1,643  304 
IL-6 (pg/ml)  2,734  1,401  ND  ND  ND 
Neutrophils (%)   81.7   54.8       83.5  69.6   22.1 
E. coli (CFU)  34.9 × 103  350 × 103  2.3 × 103  62.5 × 103  510 × 103 
LPS (EU/ml)  36.7  62.9       23.8  51.2   64.0 
+/+−/−−/− IgM−/− PtC-IgM−/− PC-IgM
TNF-α (pg/ml)  1,507  892  2,068  1,643  304 
IL-6 (pg/ml)  2,734  1,401  ND  ND  ND 
Neutrophils (%)   81.7   54.8       83.5  69.6   22.1 
E. coli (CFU)  34.9 × 103  350 × 103  2.3 × 103  62.5 × 103  510 × 103 
LPS (EU/ml)  36.7  62.9       23.8  51.2   64.0 

sIgM-deficient mice (−/−), wild-type mice (+/+), and IgM-reconstituted sIgM-deficient mice were subject to CLP. Peritoneal lavage was carried out by injection of 3 ml i.p. of PBS with 2% FCS 3 h after CLP. IgM-reconstituted mice were given a single dose of 0.5 mg i.v. of purified polyclonal IgM from normal mouse serum or monoclonal IgM specific to PtC or PC 4 h before CLP. Peritoneal lavages of sIgM-deficient and wild-type mice were each pooled from seven mice, and the levels of TNF-α, IL-6, and LPS, E. coli counts, and neutrophils were assayed (see Materials and Methods). The levels of TNF-α and LPS, E. coli counts, and neutrophils were assayed in peritoneal lavage from individual IgM-reconstituted mice. The average of four mice is shown. Similar results were obtained in a separate experiment.  

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