Tumorigenicity of C26/IL-12 and Induction of both Memory Response and CMC to Parental C26 Cells in BALB/c and GKO Mice
| Recipients . | . | No. of cells injected . | . | Primary response . | . | Secondary response* . | . | . | . | CMC on targets‡ (% lysis of target) . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | C26 . | . | F1 . | ||||||||||
| BALB/c | 5 × 104 | 10/17§ | 6/6§ | E/T | ||||||||||
| 5 × 105 | 0/17 | 1/9 | 50 | 40.7 | 12.6 | |||||||||
| 25 | 36.9 | 7.5 | ||||||||||||
| 12 | 22.4 | 5.1 | ||||||||||||
| GKO | 5 × 104 | 8/17 | 0/7 | E/T | ||||||||||
| 5 × 105 | 11/16 | 0/2 | 50 | 76.3 | 15.3 | |||||||||
| 25 | 58.8 | 10.1 | ||||||||||||
| 12 | 40.3 | 6.3 | ||||||||||||
| Recipients . | . | No. of cells injected . | . | Primary response . | . | Secondary response* . | . | . | . | CMC on targets‡ (% lysis of target) . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | C26 . | . | F1 . | ||||||||||
| BALB/c | 5 × 104 | 10/17§ | 6/6§ | E/T | ||||||||||
| 5 × 105 | 0/17 | 1/9 | 50 | 40.7 | 12.6 | |||||||||
| 25 | 36.9 | 7.5 | ||||||||||||
| 12 | 22.4 | 5.1 | ||||||||||||
| GKO | 5 × 104 | 8/17 | 0/7 | E/T | ||||||||||
| 5 × 105 | 11/16 | 0/2 | 50 | 76.3 | 15.3 | |||||||||
| 25 | 58.8 | 10.1 | ||||||||||||
| 12 | 40.3 | 6.3 | ||||||||||||
Mice that were tumor-free at day 60 were challenged subcutaneously with 105 C26 cells.
Tumor-free mice, which were not challenged, were killed and their splenocytes were tested for CMC activity in vitro. Splenocytes were restimulated in vitro with irradiated C26 cells for 5 d before CMC assay.
No. of mice with tumor/no. of mice injected.