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Table 1

Transgenic Mice Examined

AnimalSexAgeSerum IFN-γIIFStaining pattern on CKWestern blotCLENAKidney DIF
      pg/ml             
 1    9       −   −  
 2    5       −   −   
 3   12  UN           
 4    4  15             
 5    4  UN             
 6    5  40    −   −  − 
 7   12             ++ 
 8    9  UN     −     +++ 
 9    9      −   −  − 
10   12  UN            
11    7  UN    −     ++ 
12    6       −   −  
13   10             ++ 
14   10  UN          +++ 
15   11  UN            
16    4  UN     −   Sm  +++ 
17     4      −   −  − 
18   12  15        −     
19    5  30    −   −  +++ 
20    5      −  −  −  − 
21    8              
22    8      −      +++ 
23    8              
24    9              
25    5    −           
26    4    −           
27   13          −     
28    3    −           
29   13  UN             
30   12  UN             
31   12  UN             
32   12               
33    7              
34    7              
AnimalSexAgeSerum IFN-γIIFStaining pattern on CKWestern blotCLENAKidney DIF
      pg/ml             
 1    9       −   −  
 2    5       −   −   
 3   12  UN           
 4    4  15             
 5    4  UN             
 6    5  40    −   −  − 
 7   12             ++ 
 8    9  UN     −     +++ 
 9    9      −   −  − 
10   12  UN            
11    7  UN    −     ++ 
12    6       −   −  
13   10             ++ 
14   10  UN          +++ 
15   11  UN            
16    4  UN     −   Sm  +++ 
17     4      −   −  − 
18   12  15        −     
19    5  30    −   −  +++ 
20    5      −  −  −  − 
21    8              
22    8      −      +++ 
23    8              
24    9              
25    5    −           
26    4    −           
27   13          −     
28    3    −           
29   13  UN             
30   12  UN             
31   12  UN             
32   12               
33    7              
34    7              

Age is quoted in months. M, male; F, female; IIF, indirect immunofluorescence on normal mouse skin or esophagus; CK, cultured keratinocytes; CL, testing for anti-dsDNA on C. luciliae; ENA, antibodies against extractable nuclear antigens; DIF, direct immunofluorescence; UN, undetectable; N, nuclear staining on cultured mouse and/or human keratinocytes. On direct immunofluorescence of kidneys, glomerular staining intensity ranged from none (−) to mild/focal (+), moderate (++), or intense (+++). The presence of subendothelial-mesangial deposits was confirmed by electron microscopy in mice 13 and 16. There was histological evidence of severe proliferative GN in animals 12, 14, and 16 (see Table 3).  

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