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Table 2

Peptide-specific T Cell Frequencies Enumerated by IFN-γ ELISPOT and Corresponding Precursor Frequencies by LDA for Influenza Epitopes in a Series of Individuals

DonorEpitope testedHLA class I restrictionSequenceEffector frequency in PBMCs by ELISPOTPrecursor frequency in PBMCs by LDA
WB (9/96)*  M1 58–66  HLA-A2.01  GILGFVFTL  1/15,000  1/100,000 
WB (11/96)  M1 58–66  HLA-A2.01  GILGFVFTL  1/111,000  1/250,000 
SM  M1 58–66  HLA-A2.01  GILGFVFTL  1/6,000  1/59,000 
JM  M1 58–66  HLA-A2.01  GILGFVFTL  1/77,000  1/333,000 
PM  NP 380–388  HLA-B8  ELRSRYWAI  1/43,000  1/200,000 
AH  NP 265–273  HLA-A3  ILRGSVAHK  1/45,000  1/42,000 
DonorEpitope testedHLA class I restrictionSequenceEffector frequency in PBMCs by ELISPOTPrecursor frequency in PBMCs by LDA
WB (9/96)*  M1 58–66  HLA-A2.01  GILGFVFTL  1/15,000  1/100,000 
WB (11/96)  M1 58–66  HLA-A2.01  GILGFVFTL  1/111,000  1/250,000 
SM  M1 58–66  HLA-A2.01  GILGFVFTL  1/6,000  1/59,000 
JM  M1 58–66  HLA-A2.01  GILGFVFTL  1/77,000  1/333,000 
PM  NP 380–388  HLA-B8  ELRSRYWAI  1/43,000  1/200,000 
AH  NP 265–273  HLA-A3  ILRGSVAHK  1/45,000  1/42,000 

LDAs were performed as described in Materials and Methods and assayed at 14 d of culture, except where marked. Wells were scored positive if peptide-specific lysis was >10% above background. Spontaneous release was <30% of maximal release for all assays, except WB (11/96) (= 40%).  

*

 8 d LDA.  

 18 d LDA after second restimulation on day 14.  

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