Rockefeller University Press

Table I

Phenotype and Frequency of B lymphocytes in Lymphoid Tissues

Tissue	Phenotype		Ig^HEL	Ig^HEL hCD19^+/+	sHEL Ig^HEL	sHEL Ig^HEL hCD19^+/+
Tissue	Phenotype		Frequency (%) and number (No. × 10⁻⁶) of B cells^*
bone	% IgM⁻B220^lo		14 ± 2	14 ± 2	13 ± 2	13 ± 2
marrow	% IgM⁺B220^lo		32 ± 4	49 ± 10	46 ± 2	53 ± 3
	% IgM⁺B220^hi		16 ± 3	8 ± 3^§	12 ± 2	8 ± 1
	% HSA^lo B220^hi		25 ± 3	9 ± 3^‖	17 ± 5	5 ± 1^§
blood	% B220⁺		43 ± 3	7 ± 2^‖	22 ± 3	6 ± 2^‖
	# B220⁺		2.4 ± 0.5	0.3 ± 0.1^‖	0.9 ± 0.2	0.2 ± 0.1^‖
spleen	% B220⁺		36 ± 6	28 ± 5	44 ± 4	17 ± 3^‖
	# B220⁺		24 ± 5	14 ± 3^§	29 ± 5	15 ± 6^§
peritoneum	% CD5⁺ B220^lo		2.2 ± 0.3	1.3 ± 0.4	4.5 ± 1.1	1.9 ± 0.7
	# CD5⁺ B220^lo		0.05 ± 0.01	0.05 ± 0.02	0.10 ± 0.02	0.04 ± 0.02
	% CD5⁻ B220^hi		38 ± 6	2.7 ± 0.7^‖	14 ± 2	1.2 ± 0.4^‖
	# CD5⁻ B220^hi		1.0 ± 0.3	0.07 ± 0.03^§	0.33 ± 0.03	0.02 ± 0.01^‖

Expression	Source of B cells		Levels relative to Ig^HEL mice (% ± SEM)^‡
IgM levels:	bone	B220^lo	100	69 ± 12^‖	41 ± 9	22 ± 8
	marrow:	B220^hi	100	63 ± 4^‖	4.1 ± 0.5	4.3 ± 0.7
	blood:	B220⁺	100	68 ± 15^‖	19 ± 3	25 ± 4
	spleen:	B220⁺	100	62 ± 4^‖	16 ± 2	7 ± 1^‖
I-A levels:	blood:	IgM⁺	100	126 ± 5^‖	169 ± 7	254 ± 33^‖
	spleen:	IgM⁺	100	259 ± 55^§	184 ± 13	283 ± 30^§

Tissue	Phenotype		Ig^HEL	Ig^HEL hCD19^+/+	sHEL Ig^HEL	sHEL Ig^HEL hCD19^+/+
Tissue	Phenotype		Frequency (%) and number (No. × 10⁻⁶) of B cells^*
bone	% IgM⁻B220^lo		14 ± 2	14 ± 2	13 ± 2	13 ± 2
marrow	% IgM⁺B220^lo		32 ± 4	49 ± 10	46 ± 2	53 ± 3
	% IgM⁺B220^hi		16 ± 3	8 ± 3^§	12 ± 2	8 ± 1
	% HSA^lo B220^hi		25 ± 3	9 ± 3^‖	17 ± 5	5 ± 1^§
blood	% B220⁺		43 ± 3	7 ± 2^‖	22 ± 3	6 ± 2^‖
	# B220⁺		2.4 ± 0.5	0.3 ± 0.1^‖	0.9 ± 0.2	0.2 ± 0.1^‖
spleen	% B220⁺		36 ± 6	28 ± 5	44 ± 4	17 ± 3^‖
	# B220⁺		24 ± 5	14 ± 3^§	29 ± 5	15 ± 6^§
peritoneum	% CD5⁺ B220^lo		2.2 ± 0.3	1.3 ± 0.4	4.5 ± 1.1	1.9 ± 0.7
	# CD5⁺ B220^lo		0.05 ± 0.01	0.05 ± 0.02	0.10 ± 0.02	0.04 ± 0.02
	% CD5⁻ B220^hi		38 ± 6	2.7 ± 0.7^‖	14 ± 2	1.2 ± 0.4^‖
	# CD5⁻ B220^hi		1.0 ± 0.3	0.07 ± 0.03^§	0.33 ± 0.03	0.02 ± 0.01^‖

Expression	Source of B cells		Levels relative to Ig^HEL mice (% ± SEM)^‡
IgM levels:	bone	B220^lo	100	69 ± 12^‖	41 ± 9	22 ± 8
	marrow:	B220^hi	100	63 ± 4^‖	4.1 ± 0.5	4.3 ± 0.7
	blood:	B220⁺	100	68 ± 15^‖	19 ± 3	25 ± 4
	spleen:	B220⁺	100	62 ± 4^‖	16 ± 2	7 ± 1^‖
I-A levels:	blood:	IgM⁺	100	126 ± 5^‖	169 ± 7	254 ± 33^‖
	spleen:	IgM⁺	100	259 ± 55^§	184 ± 13	283 ± 30^§

Cumulative mean (± SEM) frequencies of different cell populations from at least five 2-mo-old mice of each genotype. Flow cytometry gates similar to those shown in Fig. 3 were used to determine the frequency of each cell type within the lymphocyte population. B cell numbers for blood indicate the number of cells/ml. B cell numbers from spleen and peritoneum were determined based on the total number of lymphocytes recovered.

‡

Relative cell surface antigen densities were determined by comparing the channel numbers of mean linear fluorescence intensity between IgHEL B cells and B cells fom other mice. Values represent the mean expression levels obtained from at least three sets of mice of each genotype. All samples in each set of mice were stained in parallel and analyzed sequentially by flow cytometry with identical instrument settings.

Differences between mice not expressing hCD19 and those expressing hCD19 were significant, P <0.05.

‖

P <0.01.

View Large

This Feature Is Available To Subscribers Only