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Table 1

Flow Cytometry Analysis of IL-2Prom→ IL-10cDNA–transfected T Cells*

Cell surface antigenPercentage of positive staining cells
Normal p139–151-specific T cell lineTransfected p139–151-specific T cell lineTransfected p139–151-specific T10.11 T cell clone
CD3  92.3  91.7  95.1 
CD4  94.6  93.0  99.6 
CD8   0.8   2.9   0.4 
TCR αβ  95.5  93.6  98.6 
TCR-Vβ14  14.9  ND  99.5 
CD25 (IL-2Rα)  68.8  65.6  ND 
CD44 (Pgp-1)  95.1  93.7  92.4 
CD49d (VLA-4)  45.3  42.2  41.2 
CD62L (l-selectin)  ND  20.6  19.2 
Cell surface antigenPercentage of positive staining cells
Normal p139–151-specific T cell lineTransfected p139–151-specific T cell lineTransfected p139–151-specific T10.11 T cell clone
CD3  92.3  91.7  95.1 
CD4  94.6  93.0  99.6 
CD8   0.8   2.9   0.4 
TCR αβ  95.5  93.6  98.6 
TCR-Vβ14  14.9  ND  99.5 
CD25 (IL-2Rα)  68.8  65.6  ND 
CD44 (Pgp-1)  95.1  93.7  92.4 
CD49d (VLA-4)  45.3  42.2  41.2 
CD62L (l-selectin)  ND  20.6  19.2 
*

 Two-color flow cytometry analysis of PLP 139–151-activated fixed T cells was performed with PE-conjugated rat mAb to mouse CD3 or CD4 (GIBCO BRL) and FITC-conjugated mAb to either TCR αβ chains (TCR αβ), CD25 (IL-2R α chain), CD44 (Pgp-1, CD49d (VLA-4; α4β1 integrin), or CD62L (l-selectin) (PharMingen). Single-color analysis with FITC-conjugated antibodies (PharMingen) was used for determining TCR-Vβ utilization. Isotype-matched FITC- and PE-conjugated mAb were used as controls. Data were collected on 20,000 events with a FACScan® flow cytometer. Analysis was performed on the gated lymphoblast population using Cellquest software (Becton Dickinson).  

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