IL-12–induced Upregulation of IFN-γ in Normal BALB/c Mice
| Treatment of mice . | . | IFN-γ (pg/ml) . | ||
|---|---|---|---|---|
| Supernatant . | . | Serum . | ||
| Saline/BSA | 12 ± 9 | 304 ± 271 | ||
| IL-12 | >3,000 | >3,000 | ||
| Treatment of mice . | . | IFN-γ (pg/ml) . | ||
|---|---|---|---|---|
| Supernatant . | . | Serum . | ||
| Saline/BSA | 12 ± 9 | 304 ± 271 | ||
| IL-12 | >3,000 | >3,000 | ||
Mice were given daily intraperitoneal injections of 1 μg of IL-12 for 4 d. 24 h after the last injection, serum was collected and spleen cells were harvested and cultured in vitro (5 × 106 cells/ml) for an additional 24 h with no further stimulation. Results are mean ± SEM values from three mice per group from a single experiment.