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Table 4

IL-12–induced Upregulation of IFN-γ in Normal BALB/c Mice

Treatment of miceIFN-γ (pg/ml)
SupernatantSerum
Saline/BSA  12 ± 9  304 ± 271 
IL-12  >3,000  >3,000 
Treatment of miceIFN-γ (pg/ml)
SupernatantSerum
Saline/BSA  12 ± 9  304 ± 271 
IL-12  >3,000  >3,000 

Mice were given daily intraperitoneal injections of 1 μg of IL-12 for 4 d. 24 h after the last injection, serum was collected and spleen cells were harvested and cultured in vitro (5 × 106 cells/ml) for an additional 24 h with no further stimulation. Results are mean ± SEM values from three mice per group from a single experiment.  

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