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Table 2

Expression of TNF-α mRNA in L. donovani–infected Liver

Weeks after infectionStrainTNF present no. samples/total (%)
Uninfected  GKO       0/4 (0) 
  BALB/c       0/5 (0) 
     
 2  GKO       0/6 (0) 
  BALB/c       3/6 (50) 
     
 4  GKO       3/6 (50) 
  BALB/c       5/6 (83) 
     
 8  GKO       5/6 (83) 
  BALB/c       3/6 (50) 
     
12  GKO   6/6 (100) 
  BALB/c       1/6 (17) 
Weeks after infectionStrainTNF present no. samples/total (%)
Uninfected  GKO       0/4 (0) 
  BALB/c       0/5 (0) 
     
 2  GKO       0/6 (0) 
  BALB/c       3/6 (50) 
     
 4  GKO       3/6 (50) 
  BALB/c       5/6 (83) 
     
 8  GKO       5/6 (83) 
  BALB/c       3/6 (50) 
     
12  GKO   6/6 (100) 
  BALB/c       1/6 (17) 

Qualitative RT-PCR was performed using cDNA liver samples isolated at the designated timepoints. Results are from 2–3 experiments at each timepoint.  

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