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Table 4

Reactivity of Anti–TRP-2181–188 and Anti-ovalbumin275–264 CTLs from Normal- and B16-immunized Mice with H2b-matched tumors and Peptide Pulsed Targets

IFN-γ release
Lymphocyte cultureTumor targets
NoneB16MCA 207MC-38293 + ova257–264293 + TPR-2181–188293KbDb + ova257–264293KbDb + TRP-2181–188
  U/ml* 
Normal spleen + ova257–264  36          92   8   4  35  29  74           42 
Immune spleen§ + ova257–264   1          43  50   9   9  14  91           18 
Normal spleen + TRP-2181–188   0   >600  20   9   6   3   3  >600 
Immune spleen§ + TRP-2181–188   5  >600  77  28  12  12  11  >600 
Line A T cells   0  >600  24   7   0   0   0          134 
IFN-γ release
Lymphocyte cultureTumor targets
NoneB16MCA 207MC-38293 + ova257–264293 + TPR-2181–188293KbDb + ova257–264293KbDb + TRP-2181–188
  U/ml* 
Normal spleen + ova257–264  36          92   8   4  35  29  74           42 
Immune spleen§ + ova257–264   1          43  50   9   9  14  91           18 
Normal spleen + TRP-2181–188   0   >600  20   9   6   3   3  >600 
Immune spleen§ + TRP-2181–188   5  >600  77  28  12  12  11  >600 
Line A T cells   0  >600  24   7   0   0   0          134 
*

 Line A T cells were incubated with the indicated targets for 24 h and the release of IFN-γ measured.  

 Normal “naive” splenocytes.  

§

 Donor mice previously immunized 1 × 106 irradiated B16 tumor cells and 50 μg C. parvum.  

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